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IL-10改善心脏死亡大鼠离体肺灌注后供肺功能的实验研究

陈英伦 卫栋 王梓涛 杨修成 刘明昭 戴振航 陈静瑜

陈英伦, 卫栋, 王梓涛, 等. IL-10改善心脏死亡大鼠离体肺灌注后供肺功能的实验研究[J]. 器官移植, 2021, 12(4): 421-427. doi: 10.3969/j.issn.1674-7445.2021.04.008
引用本文: 陈英伦, 卫栋, 王梓涛, 等. IL-10改善心脏死亡大鼠离体肺灌注后供肺功能的实验研究[J]. 器官移植, 2021, 12(4): 421-427. doi: 10.3969/j.issn.1674-7445.2021.04.008
Chen Yinglun, Wei Dong, Wang Zitao, et al. Experimental study on the role of IL-10 in improving donor lung function after ex vivo lung perfusion in rats of cardiac death[J]. ORGAN TRANSPLANTATION, 2021, 12(4): 421-427. doi: 10.3969/j.issn.1674-7445.2021.04.008
Citation: Chen Yinglun, Wei Dong, Wang Zitao, et al. Experimental study on the role of IL-10 in improving donor lung function after ex vivo lung perfusion in rats of cardiac death[J]. ORGAN TRANSPLANTATION, 2021, 12(4): 421-427. doi: 10.3969/j.issn.1674-7445.2021.04.008

IL-10改善心脏死亡大鼠离体肺灌注后供肺功能的实验研究

doi: 10.3969/j.issn.1674-7445.2021.04.008
基金项目: 

江苏省自然科学基金青年项目 BK20190150

详细信息
    作者简介:

    陈英伦,男,1994年生,硕士,研究方向为肺移植,Email:zjchenyinglun@163.com

    通讯作者:

    陈静瑜,男,1963年生,主任医师,研究方向为肺移植,Email:chenjy@wuxiph.com

  • 中图分类号: R617, R563

Experimental study on the role of IL-10 in improving donor lung function after ex vivo lung perfusion in rats of cardiac death

More Information
  • 摘要:   目的  探讨白细胞介素(IL)-10对心脏死亡大鼠离体肺灌注(EVLP)后供肺功能的影响。  方法  将20只成年雄性SD大鼠随机分成单纯灌注组(A组)和改良灌注组(B组),每组10只。A组采用A灌注液(灌注液未添加IL-10)进行灌注,B组采用B灌注液(灌注液中添加IL-10)进行灌注,建立大鼠心脏死亡供肺EVLP模型。比较两组供肺外观及供肺组织干重/湿重(D/W)比值、供肺功能和代谢状态、供肺形态学表现和供肺炎症标志物水平。  结果  灌注结束后,A组供肺全肺明显水肿,顺应性差,气道内涌出大量水肿液,而B组未见明显水肿,顺应性较好。与A组比较,B组肺组织D/W比值较高(P < 0.05)。两组肺静脉血氧分压均在灌注2 h时达到最高,组间比较差异无统计学意义(P > 0.05)。与A组比较,B组肺动脉压升高速度较慢,灌注结束时肺动脉压较低; 且灌注液中乳酸水平下降(均为P < 0.05)。A组肺泡结构大量破坏,细胞数量稀少,B组肺泡结构相对正常,未见明显细胞水肿。A组供肺细胞凋亡较多,B组供肺未见明显细胞凋亡现象。两组灌注4 h后单核细胞趋化蛋白(MCP)-1、IL-6水平均升高,IL-4均降低(均为P < 0.05),肿瘤坏死因子(TNF)-α、IL-1α和诱导型一氧化氮合酶(iNOS)变化无统计学意义(均为P > 0.05)。  结论  IL-10可通过减少细胞凋亡改善心脏死亡大鼠EVLP后供肺的功能。

     

  • 图  1  大鼠EVLP系统

    Figure  1.  EVLP system for rat

    图  2  两组供肺外观及供肺组织D/W比值比较

    注:A、B图示A、B两组供肺外观; C图示两组供肺组织D/W比值比较,与A组比较,aP < 0.05。

    Figure  2.  Appearance of donor lung and comparison of D/W ratio of donor lung tissues between two groups

    图  3  两组肺静脉血氧分压、肺动脉压及乳酸水平比较

    注:A图示两组肺静脉血氧分压变化(10 mmHg=1.33 kPa); B图示两组肺动脉压变化,与A组比较,aP < 0.05;C图示两组乳酸水平变化,与A组比较,aP < 0.05。

    Figure  3.  Comparison of pulmonary vein partial pressure of oxygen, pulmonary artery pressure and lactic acid level between two groups

    图  4  两组供肺的病理学表现及细胞凋亡情况

    注:A、B图示供肺组织病理学表现(HE,×100);C、D图示供肺组织细胞凋亡情况(TUNEL,×200)。

    Figure  4.  Pathological findings and cell apoptosis of donor lung in two groups

    图  5  两组供肺炎症标志物水平比较

    注:A图为两组供肺IL-1α水平比较; B图为两组供肺TNF-α水平比较; C图为两组供肺iNOS水平比较; D图为两组供肺IL-4水平比较,与灌注1 h比较,aP < 0.05;E图为两组供肺IL-6水平比较,与灌注1 h比较,aP < 0.05;F图为两组供肺MCP-1水平比较,与灌注1 h比较,aP < 0.05。

    Figure  5.  Comparison of inflammation marker levels of donor lung between two groups

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出版历程
  • 收稿日期:  2021-03-28
  • 网络出版日期:  2021-07-13
  • 刊出日期:  2021-07-15

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