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允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响

王玲 拉佈旦白拉

王玲, 拉佈旦白拉. 允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响[J]. 器官移植, 2016, 7(5): 365-369. doi: 10.3969/j.issn.1674-7445.2016.05.007
引用本文: 王玲, 拉佈旦白拉. 允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响[J]. 器官移植, 2016, 7(5): 365-369. doi: 10.3969/j.issn.1674-7445.2016.05.007
Wang Ling, LaBudanbaila. Effect of permissive hypercapnia on CD4+ and CD8+ T cells of rats with acute rejection after lung transplantation[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 365-369. doi: 10.3969/j.issn.1674-7445.2016.05.007
Citation: Wang Ling, LaBudanbaila. Effect of permissive hypercapnia on CD4+ and CD8+ T cells of rats with acute rejection after lung transplantation[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 365-369. doi: 10.3969/j.issn.1674-7445.2016.05.007

允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响

doi: 10.3969/j.issn.1674-7445.2016.05.007
详细信息
    通讯作者:

    拉佈旦白拉,E-mail:labudun1@163.com

  • 中图分类号: R617, R655.3

Effect of permissive hypercapnia on CD4+ and CD8+ T cells of rats with acute rejection after lung transplantation

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  • 摘要:   目的  探讨允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响。  方法  24只Wistar雄性大鼠和12只SD雄性大鼠配对,随机分成3组(每组6对)。对照组和治疗组分别以SD大鼠和Wistar大鼠为供、受体,同种移植组以Wistar大鼠为供、受体。采用Cuff法建立大鼠左肺原位移植急性排斥反应模型。治疗组再灌注后吸入50%的氧气和8%的二氧化碳,对照组和同种移植组再灌注后吸入50%的氧气。术后7 d采用免疫组织化学(免疫组化)方法检测移植肺组织中CD4+和CD8+T细胞的表达情况;采用流式细胞仪检测外周血中CD4+和CD8+T细胞的比例;采用酶链免疫吸附试验(ELISA)测外周血中白细胞介素(IL)-2和干扰素(IFN)-γ的水平。  结果  免疫组化结果显示,与对照组相比,治疗组和同种移植组大鼠移植肺组织中CD8+T细胞表达均明显减少。流式细胞分析结果显示,与对照组比较,治疗组和同种移植组的CD8+T细胞比例均明显降低(均为P<0.05)。ELISA结果显示,与对照组比较,治疗组和同种移植组的IL-2和IFN-γ水平均明显降低(均为P<0.05)。  结论  允许性高碳酸血症通过抑制CD8+ T细胞的增殖及CD4+ T细胞释放炎症因子,从而抑制肺移植后急性排斥反应。

     

  • 图  1  各组大鼠术后7d肺组织中CD4和CD8分子的表达(二氨基联苯胺,×40)

    注:A~C图为术后7 d肺组织内CD4+T细胞的表达;D~F图为术后7 d肺组织内CD8+T细胞的表达;A、D图为对照组,B、E图为治疗组,C、F图为同种移植组;T细胞表面的褐色颗粒即为阳性细胞

    Figure  1.  Expression of CD4 and CD8 molecules in lung tissues of rats in each group at 7 d after transplantation

    图  2  各组大鼠术后7 d外周血中淋巴细胞的流式细胞图

    注:A~C图为术后7 d肺组织内CD4+T细胞的比例;D~F图为术后7 d肺组织内CD8+T细胞的比例;A、D图为对照组,B、E图为治疗组,C、F图为同种移植组

    Figure  2.  Flow cytometry figures of peripheral blood lymphocytes of rats in each group at 7 d after transplantation

    表  1  各组大鼠外周血CD4+和CD8+T细胞比例及细胞因子水平的比较

    Table  1.   Comparison of ratios of CD4+ and CD8+ T cells and cytokine levels in peripheral blood of rats in each group

    组别 n CD4+T细胞(%) CD8+T细胞(%) IL-2(pg/ml) IFN-γ(pg/ml)
    对照组 6 33.9±8.0 27.2±7.1 48±5 60.8±8.5
    治疗组 6 29.6±3.7 14.3±3.4a 29±5a 27.5±2.4a
    同种移植组 6 30.3±1.9 13.8±1.7a 28±10a 21.5±3.8a
    注:与对照组比较,aP<0.05
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出版历程
  • 收稿日期:  2016-05-20
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2016-09-15

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