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肾移植术后巨细胞病毒感染及其与NK细胞亚群相关性的初步探讨

孙曙 刘志佳 李响 金海龙 李聪然 陈昌庆 石炳毅

孙曙, 刘志佳, 李响, 等. 肾移植术后巨细胞病毒感染及其与NK细胞亚群相关性的初步探讨[J]. 器官移植, 2020, 11(6): 685-691. doi: 10.3969/j.issn.1674-7445.2020.06.006
引用本文: 孙曙, 刘志佳, 李响, 等. 肾移植术后巨细胞病毒感染及其与NK细胞亚群相关性的初步探讨[J]. 器官移植, 2020, 11(6): 685-691. doi: 10.3969/j.issn.1674-7445.2020.06.006
Sun Shu, Liu Zhijia, Li Xiang, et al. Preliminary study of cytomegalovirus infection and its correlation with NK cell subsets after renal transplantation[J]. ORGAN TRANSPLANTATION, 2020, 11(6): 685-691. doi: 10.3969/j.issn.1674-7445.2020.06.006
Citation: Sun Shu, Liu Zhijia, Li Xiang, et al. Preliminary study of cytomegalovirus infection and its correlation with NK cell subsets after renal transplantation[J]. ORGAN TRANSPLANTATION, 2020, 11(6): 685-691. doi: 10.3969/j.issn.1674-7445.2020.06.006

肾移植术后巨细胞病毒感染及其与NK细胞亚群相关性的初步探讨

doi: 10.3969/j.issn.1674-7445.2020.06.006
基金项目: 

国家自然科学基金 81570680

详细信息
    作者简介:

    孙曙,男,1981年生,硕士,副主任医师,研究方向为器官移植与免疫耐受,Email:sunyhan309@126.com

    通讯作者:

    石炳毅,男,1951年生,主任医师,博士研究生导师,研究方向为器官移植与免疫耐受,Email:shibingyi666@126.com

  • 中图分类号: R617;R373

Preliminary study of cytomegalovirus infection and its correlation with NK cell subsets after renal transplantation

More Information
  • 摘要:   目的  探讨自然杀伤(NK)细胞亚群在肾移植术后巨细胞病毒(CMV)感染受者中的变化趋势。  方法  回顾性分析92例肾移植受者的临床资料,将肾移植受者分为CMV感染组(43例)、CMV感染恢复组(13例)、肾功能稳定组(15例)、排斥反应组(11例)和其他感染组(10例);另外选取健康成人志愿者作为健康对照组(15名)。观察并比较各组外周血NK细胞比例,及NK细胞中CD226和CD16的表达比例和平均荧光强度(MFI)。  结果  CMV感染组NK细胞比例为4.9%(2.2%,11.5%),CMV感染恢复组NK细胞比例为3.7%(2.3%,6.5%),CMV感染组和CMV感染恢复组NK细胞比例均低于其他各组(均为P < 0.05)。CMV感染组NK细胞中CD226和CD16的表达比例低于健康对照组和肾功能稳定组(均为P < 0.05)。CMV感染恢复组NK细胞中CD226和CD16的表达比例均高于CMV感染组(均为P < 0.05)。CMV感染组CD226和CD16的MFI均低于健康对照组(均为P < 0.05)。CMV感染恢复组CD226和CD16的MFI均高于CMV感染组(均为P < 0.05)。  结论  CMV感染期NK细胞中CD226和CD16的表达比例及MFI下调,而在CMV感染恢复期上调,提示NK细胞表达的CD226、CD16与CMV感染的病程密切相关。

     

  • 图  1  各组外周血NK细胞比例的比较

    Figure  1.  Comparison of the proportion of NK cells in peripheral blood among each group

    图  2  各组NK细胞中CD226和CD16表达比例的比较

    Figure  2.  Comparison of the proportion CD226 and CD16 in NK cells among each group

    图  3  CMV感染组NK细胞中CD226和CD16的流式细胞图

    注:A图为淋巴细胞分群;B图为NK细胞中CD226的表达;C图为NK细胞中CD16的表达。

    Figure  3.  Flow cytometry of CD226 and CD16 in NK cells of CMV infection group

    图  4  各组NK细胞中CD226和CD16的MFI比较

    Figure  4.  Comparison of MFI of CD226 and CD16 in NK cells among each group

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出版历程
  • 收稿日期:  2020-07-30
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2021-01-19

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