留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

异种移植中猪内源性逆转录病毒的传播

余一凡, 宋佳华, 宋相钦, 等. 异种移植中猪内源性逆转录病毒的传播[J]. 器官移植, 2023, 14(5): 754-759. doi: 10.3969/j.issn.1674-7445.2023293
引用本文: 余一凡, 宋佳华, 宋相钦, 等. 异种移植中猪内源性逆转录病毒的传播[J]. 器官移植, 2023, 14(5): 754-759. doi: 10.3969/j.issn.1674-7445.2023293
Yu Yifan, Song Jiahua, Song Xiangqin, et al. Transmission of porcine endogenous retrovirus in xenotransplantation[J]. ORGAN TRANSPLANTATION, 2023, 14(5): 754-759. doi: 10.3969/j.issn.1674-7445.2023293
Citation: Yu Yifan, Song Jiahua, Song Xiangqin, et al. Transmission of porcine endogenous retrovirus in xenotransplantation[J]. ORGAN TRANSPLANTATION, 2023, 14(5): 754-759. doi: 10.3969/j.issn.1674-7445.2023293

异种移植中猪内源性逆转录病毒的传播

doi: 10.3969/j.issn.1674-7445.2023293
基金项目: 国家自然科学基金(82260154);海南省卫生计生行业科研项目(20A200360);海南省科技厅自然科学基金(820RC766);海南医学院2021年度研究生创新科研课题(HYYS2021B0)
详细信息
    作者简介:
    通讯作者:

    王毅(ORCID:0000-0001-7152-0757),医学博士、二级教授、一级主任医师、博士研究生导师,研究方向为外科学、异种移植,Email:wayne0108@126.com

  • 中图分类号: R617, Q527+.5

Transmission of porcine endogenous retrovirus in xenotransplantation

More Information
  • 摘要: 器官移植是治疗各类终末期疾病最有效的手段,为了解决器官移植中供体短缺的问题,人们开始探究异种移植的可能。猪是异种器官移植常见的供体来源之一,猪器官作为连接两个物种间的桥梁,其携带的病毒有可能在物种间传播并存在引起人畜共患病的风险。猪内源性逆转录病毒(PERV)整合在基因组中,是一种跨物种传播的逆转录病毒。本文介绍了PERV传播特性的影响因素、PERV及其重组病毒的传播风险以及异种移植试验中PERV的检测与传播风险评估,以期为缓解器官移植供体严重短缺的现状,推动异种移植技术的发展提供参考。

     

  • [1] OPTN/SRTR 2020 annual data report: preface[J]. Am J Transplant, 2022, 22 (Suppl 2): 1-10. DOI: 10.1111/ajt.16975.
    [2] WANG Y, LEI T, WEI L, et al. Xenotransplantation in China: present status[J]. Xenotransplantation, 2019, 26(1): e12490. DOI: 10.1111/xen.12490.
    [3] COZZI E, SCHNEEBERGER S, BELLINI MI, et al. Organ transplants of the future: planning for innovations including xenotransplantation[J]. Transpl Int, 2021, 34(11): 2006-2018. DOI: 10.1111/tri.14031.
    [4] FISCHER K, SCHNIEKE A. Xenotransplantation becoming reality[J]. Transgenic Res, 2022, 31(3): 391-398. DOI: 10.1007/s11248-022-00306-w.
    [5] NIU D, MA X, YUAN T, et al. Porcine genome engineering for xenotransplantation[J]. Adv Drug Deliv Rev, 2021, 168: 229-245. DOI: 10.1016/j.addr.2020.04.001.
    [6] 张玄, 王琳, 张洪涛, 等. 多基因编辑猪-猴心脏、肝脏、肾脏移植临床前研究初步报道[J]. 器官移植, 2021, 12(1): 51-56. DOI: 10.3969/j.issn.1674-7445.2021.01.008.

    ZHANG X, WANG L, ZHANG HT, et al. Preliminary report of a preclinical study of multigene-edited pig-monkey heart, liver, and kidney transplantation [J]. Organ Transplant, 2021, 12(1): 51-56. DOI: 10.3969/j.issn.1674-7445.2021.01.008.
    [7] DENNER J. Porcine endogenous retroviruses and xenotransplantation, 2021 [J]. Viruses, 2021, 13(11): 2156. DOI: 10.3390/v13112156.
    [8] COOPER DKC, HARA H, IWASE H, et al. Pig kidney xenotransplantation: progress toward clinical trials [J]. Clin Transplant, 2021, 35(1): e14139. DOI: 10.1111/ctr.14139.
    [9] PIERSON RN 3RD. Progress toward pig-to-human xenotransplantation[J]. N Engl J Med, 2022, 386(20): 1871-1873. DOI: 10.1056/NEJMp2118019.
    [10] GRIFFITH BP, GOERLICH CE, SINGH AK, et al. Genetically modified porcine-to-human cardiac xenotransplantation [J]. N Engl J Med, 2022, 387(1): 35-44. DOI: 10.1056/NEJMoa2201422.
    [11] 马玲, 钟华, 秦树英, 等. 广西巴马小型猪内源性反转录病毒整合后传代次数对HEK293细胞中HERV-W表达和变异的影响[J]. 中国兽医学报, 2020, 40(10): 1918-1923. DOI: 10.16303/j.cnki.1005-4545.2020.10.05.

    MA L, ZHONG H, QIN SY, et al. Effects of HEK293-PERV-BM passages on the expression and variation of HERV-W in HEK293 cells[J]. Chin J Vet Sci, 2020, 40(10): 1918-1923. DOI: 10.16303/j.cnki.1005-4545.2020.10.05.
    [12] FISHMAN JA. Risks of infectious disease in xenotransplantation[J]. N Engl J Med, 2022, 387(24): 2258-2267. DOI: 10.1056/NEJMra2207462.
    [13] CARRIER AN, VERMA A, MOHIUDDIN M, et al. Xenotransplantation: a new era[J]. Front Immunol, 2022, 13: 900594. DOI: 10.3389/fimmu.2022.900594.
    [14] MEIER RPH, LONGCHAMP A, MOHIUDDIN M, et al. Recent progress and remaining hurdles toward clinical xenotransplantation[J]. Xenotransplantation, 2021, 28(3): e12681. DOI: 10.1111/xen.12681.
    [15] VAN DER LAAN LJ, LOCKEY C, GRIFFETH BC, et al. Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice[J]. Nature, 2000, 407(6800): 90-94. DOI: 10.1038/35024089.
    [16] SONG M, FITCH ZW, SAMY KP, et al. Coagulation, inflammation, and CD46 transgene expression in neonatal porcine islet xenotransplantation[J]. Xenotransplantation, 2021, 28(3): e12680. DOI: 10.1111/xen.12680.
    [17] CARVALHO-OLIVEIRA M, VALDIVIA E, BLASCZYK R, et al. Immunogenetics of xenotransplantation[J]. Int J Immunogenet, 2021, 48(2): 120-134. DOI: 10.1111/iji.12526.
    [18] KONO K, KATAOKA K, YUAN Y, et al. Infectivity assessment of porcine endogenous retrovirus using high-throughput sequencing technologies[J]. Biologicals, 2021, 71: 1-8. DOI: 10.1016/j.biologicals.2021.05.001.
    [19] LE TISSIER P, STOYE JP, TAKEUCHI Y, et al. Two sets of human-tropic pig retrovirus[J]. Nature, 1997, 389(6652): 681-682. DOI: 10.1038/39489.
    [20] DENNER J. Virus safety of xenotransplantation[J]. Viruses, 2022, 14(9): 1926. DOI: 10.3390/v14091926.
    [21] NIU D, WEI HJ, LIN L, et al. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9[J]. Science, 2017, 357(6357): 1303-1307. DOI: 10.1126/science.aan4187.
    [22] WEISS RA. Infection hazards of xenotransplantation: retrospect and prospect[J]. Xenotransplantation, 2018, 25(4): e12401. DOI: 10.1111/xen.12401.
    [23] 曾嘉庆, 高岩, 仇相书, 等. 猪逆转录病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立及应用[J]. 中国病原生物学杂志, 2022, 17(1): 26-30. DOI: 10.13350/j.cjpb.220107.

    ZENG JQ, GAO Y, QIU XS, et al. Establishment and application of a quantitative PCR assay for porcine retrovirus SYBR GreenI fluorescence [J]. Chin J Pathog Biol, 2022, 17(1): 26-30. DOI: 10.13350/j.cjpb.220107.
    [24] 冯天悦, KAMAL SHAH, 张文立, 等. 猪内源性逆转录病毒在3种不同品系猪中的分布及序列特征研究[J]. 中国预防兽医学报, 2022, 44(11): 1141-1148, 1168. DOI: 10.3969/j.issn.1008-0589.202201027.

    FENG TY, KAMAL S, ZHANG WL, et al. Distribution and sequence characteristics of porcine endogenous retroviruses in three different breeds of pigs[J]. Chin J Prev Vet Med, 2022, 44(11): 1141-1148, 1168. DOI: 10.3969/j.issn.1008-0589.202201027.
    [25] DENNER J, TÖNJES RR. Infection barriers to successful xenotransplantation focusing on porcine endogenous retroviruses[J]. Clin Microbiol Rev, 2012, 25(2): 318-343. DOI: 10.1128/CMR.05011-11.
    [26] 范瑞, 贾俊婷, 钟亚迪, 等. 五指山小型猪不同器官内猪内源性反转录病毒的存在与表达[J]. 生物技术通讯, 2018, 29(1): 50-54. DOI: 10.3969/j.issn.1009-0002.2018.01.010.

    FAN R, JIA JT, ZHONG YD, et al. Presence and expression of swine endogenous retrovirus in different organs of Wuzhishan minipigs [J]. Biotechnol Commun, 2018, 29(1): 50-54. DOI: 10.3969/j.issn.1009-0002.2018.01.010.
    [27] 白少川, 张乐超, 葛琳涵, 等. 内源性逆转录病毒的转录表达与宿主功能关系的研究进展[J]. 畜牧兽医学报, 2021, 52(8): 2107-2114. DOI: 10.11843/j.issn.0366-6964.2021.08.004.

    BAI SC, ZHANG LC, GE LH, et al. Research progress of relation between transcriptional expression of endogenous retrovirus and host function[J]. Acta Vet Et Zootech Sin, 2021, 52(8): 2107-2114. DOI: 10.11843/j.issn.0366-6964.2021.08.004.
    [28] UEDA MT, KRYUKOV K, MITSUHASHI S, et al. Comprehensive genomic analysis reveals dynamic evolution of endogenous retroviruses that code for retroviral-like protein domains[J]. Mob DNA, 2020, 11: 29. DOI: 10.1186/s13100-020-00224-w.
    [29] XUE B, SECHI LA, KELVIN DJ. Human endogenous retrovirus K (HML-2) in health and disease[J]. Front Microbiol, 2020, 11: 1690. DOI: 10.3389/fmicb.2020.01690.
    [30] 崔杰. 病毒如何与人类共进化——内源性逆转录病毒的秘密[J]. 科学(上海), 2020, 72(3): 50-53. DOI: 10.3969/j.issn.0368-6396.2020.03.012.

    CUI J. How did viruses co-evolve with humans——the secret of endogenous retroviruses [J]. Science (Shanghai), 2020, 72 (3): 50-53. DOI: 10.3969/j.issn.0368-6396.2020.03.012.
    [31] 蒋鸿涛, 李涛, 何松哲, 等. 基因修饰猪-非人灵长类动物异种肾移植面临的问题及挑战[J]. 器官移植, 2022, 13(6): 810-817. DOI: 10.3969/j.issn.1674-7445.2022.06.018.

    JIANG HT, LI T, HE SZ, et al. Problems and challenges of genetically modified pig to non-human primate kidney xenotransplantation[J]. Organ Transplant, 2022, 13(6): 810-817. DOI: 10.3969/j.issn.1674-7445.2022.06.018.
    [32] PATIENCE C, TAKEUCHI Y, WEISS RA. Infection of human cells by an endogenous retrovirus of pigs[J]. Nat Med, 1997, 3(3): 282-286. DOI: 10.1038/nm0397-282.
    [33] BACH FH, FINEBERG HV. Call for moratorium on xenotransplants[J]. Nature, 1998, 391(6665): 326. DOI: 10.1038/34766.
    [34] PARADIS K, LANGFORD G, LONG Z, et al. Search for cross-species transmission of porcine endogenous retrovirus in patients treated with living pig tissue. the XEN 111 Study Group[J]. Science, 1999, 285(5431): 1236-1241. DOI: 10.1126/science.285.5431.1236.
    [35] 邱艳, 陶开山, 李霄, 等. 猪内源性反转录病毒在异种移植中的研究进展[J/CD]. 实用器官移植电子杂志, 2018, 6(5): 405-408. DOI: 10.3969/j.issn.2095-5332.2018.05.016.

    QIU Y, TAO KS, LI X, et al. Progress of porcine endogenous retrovirus in xenotransplantation [J/CD]. Pract J Organ Transplant (Electr Vers), 2018, 6(5): 405-408. DOI: 10.3969/j.issn.2095-5332.2018.05.016.
    [36] YANG YG, WOOD JC, LAN P, et al. Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice[J]. J Clin Invest, 2004, 114(5): 695-700. DOI: 10.1172/JCI21946.
    [37] 许皓, 张玄, 窦科峰. 异种移植临床试验的有关问题[J]. 中华外科杂志, 2022, 60(10): 888-893. DOI: 10.3760/cma.j.cn112139-20220626-00286.

    XU H, ZHANG X, DOU KF. Issues related to clinical xenograft trials [J]. Chin J Surg, 2022, 60(10): 888-893. DOI: 10.3760/cma.j.cn112139-20220626-00286.
    [38] YOON CH, CHOI HJ, KIM MK. Corneal xenotransplantation: where are we standing? [J]. Prog Retin Eye Res, 2021, 80: 100876. DOI: 10.1016/j.preteyeres.2020.100876.
    [39] PISANO MP, GRANDI N, TRAMONTANO E. High-throughput sequencing is a crucial tool to investigate the contribution of human endogenous retroviruses (HERVs) to human biology and development[J]. Viruses, 2020, 12(6): 633. DOI: 10.3390/v12060633.
    [40] KRÜGER L, STILLFRIED M, PRINZ C, et al. Copy number and prevalence of porcine endogenous retroviruses (PERVs) in German wild boars[J]. Viruses, 2020, 12(4): 419. DOI: 10.3390/v12040419.
    [41] KRÜGER L, BÖTTGER J, HUANG CA, et al. Absence of porcine endogenous retrovirus (PERV) production from pig lymphoma cell lines[J]. Virus Res, 2021, 295: 198286. DOI: 10.1016/j.virusres.2020.198286.
    [42] DENNER J, SCOBIE L. Are there better assays to evaluate the risk of transmission of porcine endogenous retroviruses (PERVs) to human cells? [J]. Xenotransplantation, 2019, 26(4): e12510. DOI: 10.1111/xen.12510.
    [43] NEGRI A, WILSON L. Future systems of xenotransplantation: melding historical and bioethical methodology[J]. Cell Transplant, 2023, 32: 9636897231170510. DOI: 10.1177/09636897231170510.
  • 加载中
图(1)
计量
  • 文章访问数:  481
  • HTML全文浏览量:  322
  • PDF下载量:  63
  • 被引次数: 0
出版历程
  • 收稿日期:  2023-04-30
  • 录用日期:  2023-07-10
  • 网络出版日期:  2023-07-20
  • 刊出日期:  2023-09-15

目录

    /

    返回文章
    返回