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人源化基因修饰猪红细胞与人血清免疫相容性的体外研究

陈蕾佳, 崔梦一, 宋翔宇, 等. 人源化基因修饰猪红细胞与人血清免疫相容性的体外研究[J]. 器官移植. doi: 10.3969/j.issn.1674-7445.2023226
引用本文: 陈蕾佳, 崔梦一, 宋翔宇, 等. 人源化基因修饰猪红细胞与人血清免疫相容性的体外研究[J]. 器官移植. doi: 10.3969/j.issn.1674-7445.2023226
Chen Leijia, Cui Mengyi, Song Xiangyu, et al. In vitro study of immunocompatibility of humanized genetically modified pig erythrocytes with human serum[J]. ORGAN TRANSPLANTATION. doi: 10.3969/j.issn.1674-7445.2023226
Citation: Chen Leijia, Cui Mengyi, Song Xiangyu, et al. In vitro study of immunocompatibility of humanized genetically modified pig erythrocytes with human serum[J]. ORGAN TRANSPLANTATION. doi: 10.3969/j.issn.1674-7445.2023226

人源化基因修饰猪红细胞与人血清免疫相容性的体外研究

doi: 10.3969/j.issn.1674-7445.2023226
基金项目: 国家重点研发计划(2019YFA0110704)
详细信息
    作者简介:
    通讯作者:

    赵亚群(ORCID 0000-0001-8318-2660),医学博士,主任医师,研究方向为神经外科学、颅脑创伤等,Email:zhaoyaqun2@sina.com;彭江(ORCID 0000-0003-4662-9288),医学博士,研究员,研究方向为骨科学、组织工程技术修复骨与神经再生等,Email:pengjiang301@126.com

  • 中图分类号: R457.1, R392

In vitro study of immunocompatibility of humanized genetically modified pig erythrocytes with human serum

More Information
  • 摘要:   目的   探讨野生型(WT)、四基因修饰(TKO/hCD55)和六基因修饰(TKO/hCD55/hCD46/hTBM)猪红细胞与人血清的免疫相容性和免疫差异。  方法  收集了20名不同血型志愿者的血液,将WT、TKO/hCD55和TKO/hCD55/hCD46/hTBM猪红细胞、ABO-相容(ABO-C)及ABO-不相容(ABO-I)人红细胞分别暴露于不同血型人血清中,检测血凝集、抗原抗体结合(IgG、IgM)水平和补体依赖性细胞毒性,评估2种基因修饰猪红细胞与人血清的免疫相容性。  结果  ABO-C组未出现明显凝集;WT组和ABO-I组凝集水平高于TKO/hCD55组和TKO/hCD55/hCD46/hTBM组(均为P<0.001)。WT组猪红细胞裂解水平高于ABO-C组、TKO/hCD55组和TKO/hCD55/hCD46/hTBM组;ABO-I组猪红细胞裂解水平高于TKO/hCD55组、TKO/hCD55/hCD46/hTBM组(均为P<0.01)。TKO/hCD55组猪红细胞IgM和IgG结合水平均低于WT组和ABO-I组;TKO/hCD55/hCD46/hTBM组猪红细胞IgG和IgM结合水平低于WT组,IgG低于ABO-I组(均为P<0.05)。  结论  基因修饰猪红细胞的免疫相容性优于野生型猪,并接近于ABO-C,人源化猪红细胞在血资源奇缺时可考虑作为血液来源。

     

  • 图  1  血凝集试验

    注:A图为血凝集试验图像;B图为应用改良Marsh评分计算凝集水平,与ABO-I组比较,aP<0.001;与WT组比较,bP<0.001。

    Figure  1.  Hemagglutination assay

    图  2  补体依赖性细胞毒性实验

    注:与ABO-I组比较,aP<0.01;与WT组比较,bP<0.01。

    Figure  2.  Complement-dependent cytotoxicity assay

    图  3  红细胞的抗原抗体结合实验

    注:A图为IgG结合水平;B图为IgM结合水平。与ABO-I组比较,aP<0.01;与WT组比较,bP<0.01。

    Figure  3.  Antigen-antibody binding assay of erythrocytes

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  • 收稿日期:  2023-12-08
  • 网络出版日期:  2024-02-29

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