留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

免疫组库测序在实体器官移植中的应用

梁厉飞, 陈婷婷, 杨橙. 免疫组库测序在实体器官移植中的应用[J]. 器官移植, 2024, 15(2): 207-213. doi: 10.3969/j.issn.1674-7445.2023192
引用本文: 梁厉飞, 陈婷婷, 杨橙. 免疫组库测序在实体器官移植中的应用[J]. 器官移植, 2024, 15(2): 207-213. doi: 10.3969/j.issn.1674-7445.2023192
Liang Lifei, Chen Tingting, Yang Cheng. Application of immune repertoire sequencing in solid organ transplantation[J]. ORGAN TRANSPLANTATION, 2024, 15(2): 207-213. doi: 10.3969/j.issn.1674-7445.2023192
Citation: Liang Lifei, Chen Tingting, Yang Cheng. Application of immune repertoire sequencing in solid organ transplantation[J]. ORGAN TRANSPLANTATION, 2024, 15(2): 207-213. doi: 10.3969/j.issn.1674-7445.2023192

免疫组库测序在实体器官移植中的应用

doi: 10.3969/j.issn.1674-7445.2023192
基金项目: 国家自然科学基金(82170765);上海市青年拔尖人才开发计划;上海市临床重点专科(shslczdzk05802)
详细信息
    作者简介:
    通讯作者:

    杨橙(ORCID 0000-0003-4060-2746),Email:esuperyc@163.com

  • 中图分类号: R617, R392

Application of immune repertoire sequencing in solid organ transplantation

More Information
  • 摘要: 免疫组库一般指T细胞和B细胞的总称,它有着巨大的多样性,使免疫系统能够对多种抗原刺激做出反应。随着测序技术的发展,免疫组库测序可以从基因水平深入了解排斥反应发生时淋巴细胞克隆的变化,也为基于免疫组库测序的新型无创诊断技术的产生提供了可能。近年来,免疫组库测序在实体器官移植中的尝试不断增多,特别是在肾移植、肝移植、心脏移植以及移植后感染等领域。本文对以上领域中应用免疫组库测序的研究进行了综述,总结器官移植中免疫组库测序使用的现状和作为早期无创诊断排斥反应的新技术的潜力,以期为这项技术进一步发展并应用于临床提供借鉴。

     

  • FIG. 2990.  FIG. 2990.

    FIG. 2990..  FIG. 2990.

  • [1] 付钰, 杨国胜, 吴松. 免疫组库的临床应用[J]. 国际免疫学杂志, 2014, 37(5): 411-414,423. DOI: 10.3760/cma.j.issn.1673-4394.2014.05.012.

    FU Y, YANG GS, WU S. Immune repertoire in clinic application[J]. Int J Immunol, 2014, 37(5): 411-414,423. DOI: 10.3760/cma.j.issn.1673-4394.2014.05.012.
    [2] MUSVOSVI M, HUANG H, WANG C, et al. T cell receptor repertoires associated with control and disease progression following Mycobacterium tuberculosis infection[J]. Nat Med, 2023, 29(1): 258-269. DOI: 10.1038/s41591-022-02110-9.
    [3] FRANK ML, LU K, ERDOGAN C, et al. T-cell receptor repertoire sequencing in the era of cancer immunotherapy[J]. Clin Cancer Res, 2023, 29(6): 994-1008. DOI: 10.1158/1078-0432.CCR-22-2469.
    [4] PAUKEN KE, LAGATTUTA KA, LU BY, et al. TCR-sequencing in cancer and autoimmunity: barcodes and beyond[J]. Trends Immunol, 2022, 43(3): 180-194. DOI: 10.1016/j.it.2022.01.002.
    [5] WONG P, CINA DP, SHERWOOD KR, et al. Clinical application of immune repertoire sequencing in solid organ transplant[J]. Front Immunol, 2023, 14: 1100479. DOI: 10.3389/fimmu.2023.1100479.
    [6] OELLERICH M, SHERWOOD K, KEOWN P, et al. Liquid biopsies: donor-derived cell-free DNA for the detection of kidney allograft injury[J]. Nat Rev Nephrol, 2021, 17(9): 591-603. DOI: 10.1038/s41581-021-00428-0.
    [7] SCHINSTOCK CA, MANNON RB, BUDDE K, et al. Recommended treatment for antibody-mediated rejection after kidney transplantation: the 2019 expert consensus from the Transplantion Society Working Group[J]. Transplantation, 2020, 104(5): 911-922. DOI: 10.1097/TP.0000000000003095.
    [8] JIN Y, GAO P, LIANG L, et al. Noninvasive quantification of granzyme B in cardiac allograft rejection using targeted ultrasound imaging[J]. Front Immunol, 2023, 14: 1164183. DOI: 10.3389/fimmu.2023.1164183.
    [9] GAO T, YI L, WANG Y, et al. Granzyme B-responsive fluorescent probe for non-invasive early diagnosis of transplant rejection[J]. Biosens Bioelectron, 2023, 232: 115303. DOI: 10.1016/j.bios.2023.115303.
    [10] LIU X, WU J. History, applications, and challenges of immune repertoire research[J]. Cell Biol Toxicol, 2018, 34(6): 441-457. DOI: 10.1007/s10565-018-9426-0.
    [11] SIU JHY, SURENDRAKUMAR V, RICHARDS JA, et al. T cell allorecognition pathways in solid organ transplantation[J]. Front Immunol, 2018, 9: 2548. DOI: 10.3389/fimmu.2018.02548.
    [12] DEWOLF S, SYKES M. Alloimmune T cells in transplantation[J]. J Clin Invest, 2017, 127(7): 2473-2481. DOI: 10.1172/JCI90595.
    [13] DAVIS MM, BJORKMAN PJ. T-cell antigen receptor genes and T-cell recognition[J]. Nature, 1988, 334(6181): 395-402. DOI: 10.1038/334395a0.
    [14] PINEDA S, SIGDEL TK, LIBERTO JM, et al. Characterizing pre-transplant and post-transplant kidney rejection risk by B cell immune repertoire sequencing[J]. Nat Commun, 2019, 10(1): 1906. DOI: 10.1038/s41467-019-09930-3.
    [15] BRACIAK TA, PEDERSEN B, CHIN J, et al. Protection against experimental autoimmune encephalomyelitis generated by a recombinant adenovirus vector expressing the V beta 8.2 TCR is disrupted by coadministration with vectors expressing either IL-4 or -10[J]. J Immunol, 2003, 170(2): 765-774. DOI: 10.4049/jimmunol.170.2.765.
    [16] SCHATZ DG, OETTINGER MA, SCHLISSEL MS. V(D)J recombination: molecular biology and regulation[J]. Annu Rev Immunol, 1992, 10: 359-383. DOI: 10.1146/annurev.iy.10.040192.002043.
    [17] ALT FW, OLTZ EM, YOUNG F, et al. VDJ recombination[J]. Immunol Today, 1992, 13(8): 306-314. DOI: 10.1016/0167-5699(92)90043-7.
    [18] LIU H, PAN W, TANG C, et al. The methods and advances of adaptive immune receptors repertoire sequencing[J]. Theranostics, 2021, 11(18): 8945-8963. DOI: 10.7150/thno.61390.
    [19] FU J, KHOSRAVI-MAHARLOOEI M, SYKES M. High throughput human T cell receptor sequencing: a new window into repertoire establishment and alloreactivity[J]. Front Immunol, 2021, 12: 777756. DOI: 10.3389/fimmu.2021.777756.
    [20] 张家兴, 陈怀周, 赖柳生, 等. 肾移植术后急性排斥反应相关的T细胞受体免疫组库变化[J]. 中华肾脏病杂志, 2018, 34(4): 281-287. DOI: 10.3760/cma.j.issn.1001-7097.2018.04.009.

    ZHANG JX, CHEN HZ, LAI LS, et al. Study of T cell receptor in patients with acute rejection after renal transplantation[J]. Chin J Nephrol, 2018, 34(4): 281-287. DOI: 10.3760/cma.j.issn.1001-7097.2018.04.009.
    [21] ASCHAUER C, JELENCSICS K, HU K, et al. Effects of reduced-dose anti-human T-lymphocyte globulin on overall and donor-specific T-cell repertoire reconstitution in sensitized kidney transplant recipients[J]. Front Immunol, 2022, 13: 843452. DOI: 10.3389/fimmu.2022.843452.
    [22] ASCHAUER C, JELENCSICS K, HU K, et al. Prospective tracking of donor-reactive T-cell clones in the circulation and rejecting human kidney allografts[J]. Front Immunol, 2021, 12: 750005. DOI: 10.3389/fimmu.2021.750005.
    [23] LAI L, WANG L, CHEN H, et al. T cell repertoire following kidney transplantation revealed by high-throughput sequencing[J]. Transpl Immunol, 2016, 39: 34-45. DOI: 10.1016/j.trim.2016.08.006.
    [24] FU J, RUST D, FANG Z, et al. T cell repertoire profiling in allografts and native tissues in recipients with COVID-19 after solid organ transplantation: insight into T cell-mediated allograft protection from viral infection[J]. Front Immunol, 2022, 13: 1056703. DOI: 10.3389/fimmu.2022.1056703.
    [25] SHI T, BURG AR, CALDWELL JT, et al. Single-cell transcriptomic analysis of renal allograft rejection reveals insights into intragraft TCR clonality[J]. J Clin Invest, 2023, 133(14): e170191. DOI: 10.1172/JCI170191.
    [26] DUNLAP GS, DITORO D, HENDERSON J, et al. Clonal dynamics of alloreactive T cells in kidney allograft rejection after anti-PD-1 therapy[J]. Nat Commun, 2023, 14(1): 1549. DOI: 10.1038/s41467-023-37230-4.
    [27] SIGDEL TK, FIELDS PA, LIBERTO J, et al. Perturbations of the T-cell immune repertoire in kidney transplant rejection[J]. Front Immunol, 2022, 13: 1012042. DOI: 10.3389/fimmu.2022.1012042.
    [28] ALACHKAR H, MUTONGA M, KATO T, et al. Quantitative characterization of T-cell repertoire and biomarkers in kidney transplant rejection[J]. BMC Nephrol, 2016, 17(1): 181. DOI: 10.1186/s12882-016-0395-3.
    [29] ZHENG B, YANG Y, CHEN L, et al. B-cell receptor repertoire sequencing: deeper digging into the mechanisms and clinical aspects of immune-mediated diseases[J]. iScience, 2022, 25(10): 105002. DOI: 10.1016/j.isci.2022.105002.
    [30] WU TH, LIAO HT, LI TH, et al. High-throughput sequencing of complementarity determining region 3 in the heavy chain of B-cell receptor in renal transplant recipients: a preliminary report[J]. J Clin Med, 2022, 11(11): 2980. DOI: 10.3390/jcm11112980.
    [31] WANG G, SUI W, XUE W, et al. Comprehensive analysis of B and T cell receptor repertoire in patients after kidney transplantation by high-throughput sequencing[J]. Clin Immunol, 2022, 245: 109162. DOI: 10.1016/j.clim.2022.109162.
    [32] FERDMAN J, PORCHERAY F, GAO B, et al. Expansion and somatic hypermutation of B-cell clones in rejected human kidney grafts[J]. Transplantation, 2014, 98(7): 766-772. DOI: 10.1097/TP.0000000000000124.
    [33] YANG G, OU M, CHEN H, et al. Characteristic analysis of TCR β-chain CDR3 repertoire for pre- and post-liver transplantation[J]. Oncotarget, 2018, 9(77): 34506-34519. DOI: 10.18632/oncotarget.26138.
    [34] SAVAGE TM, SHONTS BA, LAU S, et al. Deletion of donor-reactive T cell clones after human liver transplant[J]. Am J Transplant, 2020, 20(2): 538-545. DOI: 10.1111/ajt.15592.
    [35] SHAN Y, QI D, ZHANG L, et al. Single-cell RNA-seq revealing the immune features of donor liver during liver transplantation[J]. Front Immunol, 2023, 14: 1096733. DOI: 10.3389/fimmu.2023.1096733.
    [36] MEDERACKE YS, NIENEN M, JAREK M, et al. T cell receptor repertoires within liver allografts are different to those in the peripheral blood[J]. J Hepatol, 2021, 74(5): 1167-1175. DOI: 10.1016/j.jhep.2020.12.014.
    [37] ZUBER J, SHONTS B, LAU SP, et al. Bidirectional intragraft alloreactivity drives the repopulation of human intestinal allografts and correlates with clinical outcome[J]. Sci Immunol, 2016, 1(4): eaah3732. DOI: 10.1126/sciimmunol.aah3732.
    [38] VOLLMERS C, DE VLAMINCK I, VALANTINE HA, et al. Monitoring pharmacologically induced immunosuppression by immune repertoire sequencing to detect acute allograft rejection in heart transplant patients: a proof-of-concept diagnostic accuracy study[J]. PLoS Med, 2015, 12(10): e1001890. DOI: 10.1371/journal.pmed.1001890.
    [39] KIM JY, LEI Z, MAIENSCHEIN-CLINE M, et al. Longitudinal analysis of the T-cell receptor repertoire in graft-infiltrating lymphocytes following hand transplantation[J]. Transplantation, 2021, 105(7): 1502-1509. DOI: 10.1097/TP.0000000000003535.
    [40] SIM BZ, YONG KY, SLAVIN MA, et al. Risk factors and outcome of concurrent and sequential multiviral cytomegalovirus, Epstein-Barr virus, BK virus, adenovirus and other viral reactivations in transplantation[J]. Curr Opin Infect Dis, 2022, 35(6): 536-544. DOI: 10.1097/QCO.0000000000000888.
    [41] STRANAVOVA L, PELAK O, SVATON M, et al. Heterologous cytomegalovirus and allo-reactivity by shared T cell receptor repertoire in kidney transplantation[J]. Front Immunol, 2019, 10: 2549. DOI: 10.3389/fimmu.2019.02549.
    [42] SMITH C, CORVINO D, BEAGLEY L, et al. T cell repertoire remodeling following post-transplant T cell therapy coincides with clinical response[J]. J Clin Invest, 2019, 129(11): 5020-5032. DOI: 10.1172/JCI128323.
    [43] NGUYEN TH, BIRD NL, GRANT EJ, et al. Maintenance of the EBV-specific CD8+ TCRαβ repertoire in immunosuppressed lung transplant recipients[J]. Immunol Cell Biol, 2017, 95(1): 77-86. DOI: 10.1038/icb.2016.71.
    [44] BORRIELLO M, INGROSSO D, PERNA AF, et al. BK virus infection and BK-virus-associated nephropathy in renal transplant recipients[J]. Genes (Basel), 2022, 13(7): 1290. DOI: 10.3390/genes13071290.
    [45] STERVBO U, NIENEN M, WEIST BJD, et al. BKV clearance time correlates with exhaustion state and T-cell receptor repertoire shape of BKV-specific T-cells in renal transplant patients[J]. Front Immunol, 2019, 10: 767. DOI: 10.3389/fimmu.2019.00767.
  • 加载中
图(1)
计量
  • 文章访问数:  231
  • HTML全文浏览量:  130
  • PDF下载量:  48
  • 被引次数: 0
出版历程
  • 收稿日期:  2023-11-07
  • 录用日期:  2024-01-17
  • 网络出版日期:  2024-01-24
  • 刊出日期:  2024-03-15

目录

    /

    返回文章
    返回