满霖, 李小杉, 王文静, 等. 宏基因组二代测序在实体器官移植感染防控中的应用[J]. 器官移植, 2024, 15(2): 289-296. DOI: 10.3969/j.issn.1674-7445.2023181
引用本文: 满霖, 李小杉, 王文静, 等. 宏基因组二代测序在实体器官移植感染防控中的应用[J]. 器官移植, 2024, 15(2): 289-296. DOI: 10.3969/j.issn.1674-7445.2023181
Man Lin, Li Xiaoshan, Wang Wenjing, et al. Application of metagenomic next-generation sequencing in prevention and control of infection in solid organ transplantation[J]. ORGAN TRANSPLANTATION, 2024, 15(2): 289-296. DOI: 10.3969/j.issn.1674-7445.2023181
Citation: Man Lin, Li Xiaoshan, Wang Wenjing, et al. Application of metagenomic next-generation sequencing in prevention and control of infection in solid organ transplantation[J]. ORGAN TRANSPLANTATION, 2024, 15(2): 289-296. DOI: 10.3969/j.issn.1674-7445.2023181

宏基因组二代测序在实体器官移植感染防控中的应用

Application of metagenomic next-generation sequencing in prevention and control of infection in solid organ transplantation

  • 摘要: 器官移植已成为多种终末期疾病的有效治疗手段,但器官移植受者术后需长期服用免疫抑制药,导致免疫功能低下,使得细菌、病毒和真菌感染的发生率相对较高。以病原学培养、免疫学检测和聚合酶链反应为代表的传统微生物检测方法被广泛用于感染检测,但存在耗时长、需预先假定病原体等问题。宏基因组二代测序由于具有病原体检出率高、对病原谱检测全面的优点,近年来被广泛应用于器官移植领域的感染防控。本文就宏基因组二代测序技术在实体器官移植感染防控上的应用现状进行综述,以期对移植相关感染的诊断和治疗提供参考。

     

    Abstract: Organ transplantation has become an effective treatment for multiple end-stage diseases. However, the recipients of organ transplantation need to take immunosuppressive drugs for a long time after operation, which leads to low immune function and relatively high incidence of bacterial, viral and fungal infections. Traditional microbial detection methods, such as pathogen culture, immunological detection and polymerase chain reaction, have been widely applied in infection detection, whereas these methods may cause problems, such as long detection time and presumed pathogens. Metagenomic next-generation sequencing has been widely adopted in infection prevention and control in organ transplantation in recent years due to high detection rate and comprehensive detection of pathogen spectrum. In this article, the application of metagenomic next-generation sequencing in the prevention and control of infection in solid organ transplantation was reviewed, aiming to provide reference for the diagnosis and treatment of transplantation-related infection.

     

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