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低温机械灌注对大鼠肾脏炎症因子表达水平的影响

罗德, 刘江, 周鹏程, 等. 低温机械灌注对大鼠肾脏炎症因子表达水平的影响[J]. 器官移植, 2023, 14(6): 824-830. doi: 10.3969/j.issn.1674-7445.2023132
引用本文: 罗德, 刘江, 周鹏程, 等. 低温机械灌注对大鼠肾脏炎症因子表达水平的影响[J]. 器官移植, 2023, 14(6): 824-830. doi: 10.3969/j.issn.1674-7445.2023132
Luo De, Liu Jiang, Zhou Pengcheng, et al. Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney[J]. ORGAN TRANSPLANTATION, 2023, 14(6): 824-830. doi: 10.3969/j.issn.1674-7445.2023132
Citation: Luo De, Liu Jiang, Zhou Pengcheng, et al. Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney[J]. ORGAN TRANSPLANTATION, 2023, 14(6): 824-830. doi: 10.3969/j.issn.1674-7445.2023132

低温机械灌注对大鼠肾脏炎症因子表达水平的影响

doi: 10.3969/j.issn.1674-7445.2023132
基金项目: 四川省科技厅联合创新项目(2022YFS0632-B4)
详细信息
    作者简介:
    通讯作者:

    苏松(ORCID:0000-0002-0894-1361),博士,研究方向为器官移植与免疫,Email:13882778554@163.com

  • 中图分类号: R617, R364.5

Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney

More Information
  • 摘要:   目的   探讨低温机械灌注(HMP)对大鼠肾脏炎症因子表达水平的影响。  方法  雄性大鼠30只,随机分为对照组(Control组),静态冷保存组(SCS组)和HMP组,每组10只。记录HMP过程中流速、肾内阻力和灌注流出液pH值。收集每组肾脏组织,采用逆转录聚合酶链反应(RT-PCR)检测CXC趋化因子配体(CXCL)1、CXCL2、干扰素(IFN)-β1、IFN-α4、CC趋化因子配体(CCL)2、CCL20、白细胞介素(IL)-17α、IL-17C和肿瘤坏死因子(TNF)-α的信使RNA(mRNA)表达水平,苏木素-伊红(HE)染色观察肾脏组织病理学改变。  结果  HMP过程中的流速、肾内阻力保持稳定,灌注流出液pH值缓慢降低。RT-PCR结果显示,与Control组比较,SCS组和HMP组CXCL1、CXCL2、CCL2、CCL20、IL-17α、IL-17C和TNF-α的mRNA相对表达量升高;与SCS组比较,HMP组CXCL1、CXCL2、CCL2、CCL20、IL-17α和TNF-α的mRNA相对表达量升高(均为P<0.05)。HE染色结果显示,Control组肾脏细胞形态正常,SCS组出现明显的上皮坏死、胞质空泡化、刷状缘丢失、上皮脱落,与SCS组比较,HMP组病理改变程度减轻。  结论  HMP能激活肾脏炎症反应,抑制HMP过程中的炎症反应激活有望进一步提高移植物保存效果。

     

  • 图  1  HMP过程中各参数变化

    注:A图为HMP过程中流速的变化;B图为HMP过程中肾内阻力的变化;C图为HMP过程中静脉流出液pH值的变化。

    Figure  1.  Changes of parameters during HMP

    图  2  各组肾脏炎症因子mRNA表达水平

    注:与Control组比较,aP< 0.05;与SCS组比较,bP< 0.05。

    Figure  2.  mRNA expression levels of inflammatory factors of kidneys in each group

    图  3  各组肾脏组织病理学改变(HE,×400)

    注:A图为Control组;B图为SCS组;C图为HMP组。绿色箭头为上皮坏死,黑色箭头为刷状缘丢失,蓝色箭头为胞质空泡化,红色箭头为上皮脱落。

    Figure  3.  Histopathological changes of the kidney tissues in each group

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出版历程
  • 收稿日期:  2023-07-05
  • 录用日期:  2023-09-11
  • 网络出版日期:  2023-09-19
  • 刊出日期:  2023-11-09

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