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重组人HMGB1调控内皮细胞成血管作用的机制初探

邓金涛, 许文斌, 任建华, 等. 重组人HMGB1调控内皮细胞成血管作用的机制初探[J]. 器官移植, 2023, 14(3): 397-403. doi: 10.3969/j.issn.1674-7445.2023.03.011
引用本文: 邓金涛, 许文斌, 任建华, 等. 重组人HMGB1调控内皮细胞成血管作用的机制初探[J]. 器官移植, 2023, 14(3): 397-403. doi: 10.3969/j.issn.1674-7445.2023.03.011
Deng Jintao, Xu Wenbin, Ren Jianhua, et al. Mechanism of regulatory effect of recombinant human HMGB1 on endothelial cell angiogenesis[J]. ORGAN TRANSPLANTATION, 2023, 14(3): 397-403. doi: 10.3969/j.issn.1674-7445.2023.03.011
Citation: Deng Jintao, Xu Wenbin, Ren Jianhua, et al. Mechanism of regulatory effect of recombinant human HMGB1 on endothelial cell angiogenesis[J]. ORGAN TRANSPLANTATION, 2023, 14(3): 397-403. doi: 10.3969/j.issn.1674-7445.2023.03.011

重组人HMGB1调控内皮细胞成血管作用的机制初探

doi: 10.3969/j.issn.1674-7445.2023.03.011
基金项目: 

广东省自然科学基金 2021A1515011772

详细信息
    作者简介:
    通讯作者:

    梁堂钊(ORCID:0000-0001-6059-0784),博士,副主任医师,硕士研究生导师,研究方向为骨缺损修复、3D生物打印,Email:liangtzh5@mail.sysu.edu.cn

  • 中图分类号: R617, R816.8

Mechanism of regulatory effect of recombinant human HMGB1 on endothelial cell angiogenesis

More Information
  • 摘要:   目的  探讨重组人高迁移率族蛋白1(rhHMGB1)在调控内皮细胞成血管过程中可能涉及的机制。  方法  将内皮细胞分为对照组、骨髓间充质干细胞(MSC)上清组以及rhHMGB1组。采用细胞计数试剂(CCK)-8法检测内皮细胞的增殖、存活情况;采用蛋白质印迹法检测血管内皮生长因子(VEGF)、Yes相关蛋白(YAP)、CD31、缺氧诱导因子(HIF)-1α的蛋白相对表达量;采用实时荧光定量聚合酶链反应(RT-qPCR)检测VEGF、YAP、CD31、HIF-1α的信使RNA(mRNA)相对表达量;采用Transwell实验检测内皮细胞迁移水平;免疫荧光实验检测YAP定位情况。  结果  与对照组比较,rhHMGB1组内皮细胞迁移率增加(P < 0.05),且细胞迁移率随着时间的延长而增加。与对照组比较,MSC上清组VEGF蛋白相对表达量增加,p-YAP蛋白表达增多,差异均有统计学意义(均为P < 0.05)。与对照组比较,rhHMGB1组的VEGF和HIF-1α蛋白相对表达量以及VEGF和CD31 mRNA相对表达量均增加,YAP和p-YAP蛋白表达均增多,YAP/p-YAP比值增加,差异均有统计学意义(均为P < 0.05)。与MSC上清组比较,rhHMGB1组的CD31 mRNA相对表达量增加,YAP蛋白表达增多,YAP/p-YAP比值增加,差异均有统计学意义(均为P < 0.05)。  结论  外源高浓度rhHMGB1可能通过介导YAP入核促进内皮细胞的迁移能力,并上调血管生成相关蛋白的表达。

     

  • 图  1  rhHMGB1对内皮细胞增殖和迁移能力的影响

    注:A图为各组内皮细胞增殖情况;B图为Transwell实验示各组细胞迁移情况(结晶紫,×100);C图为各组内皮细胞迁移率的统计结果。与对照组比较,aP < 0.05。

    Figure  1.  Effect of rhHMGB1 on proliferation and migration of endothelial cells

    图  2  rhHMGB1对内皮细胞VEGF、HIF-1a、CD31、YAP的mRNA和蛋白表达的影响

    注:A图为蛋白质印迹法检测各组VEGF、HIF-1α、CD31、YAP蛋白表达;B图为各组VEGF、HIF-1α、CD31、YAP蛋白相对表达量统计结果;C~F图为各组VEGF、HIF-1a、CD31、YAP mRNA相对表达量。与对照组比较,aP < 0.05;与MSC上清组比较,bP < 0.05。

    Figure  2.  Effect of rhHMGB1 on the expression levels of VEGF, HIF-1a, CD31 and YAP mRNA and protein in endothelial cells

    图  3  rhHMGB1对YAP入核的影响

    注:A图为免疫荧光示YAP定位情况(×400);B~D图为YAP、p-YAP及YAP/p-YAP比值统计结果。与对照组比较,aP < 0.05;与MSC上清组比较,bP < 0.05。

    Figure  3.  Effect of rhHMGB1 on YAP entry into the nucleus

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出版历程
  • 收稿日期:  2022-03-06
  • 刊出日期:  2023-05-15

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