Abstract: In order to promote the standardized application of ex vivo hypothermic machine perfusion preservation technology for donor kidney from deceased organ donation, Branch of Organ Transplantation of Chinese Medical Association organized the formulation of the " Guidelines for ex vivo hypothermic machine perfusion preservation of donor kidney from deceased organ donation in China". This guideline integrates expert consensus from the fields of kidney transplantation and deceased organ donation, based on existing clinical guidelines, systematic reviews, case studies, expert consensus, and other materials, and combined with clinical issues related to ex vivo hypothermic machine perfusion preservation technology for kidneys from deceased organ donation in recent years both domestically and internationally. After multiple expert discussions and reaching a consensus, the guideline was completed. The guideline contains 11 clinical questions and 14 recommendations, and the strength of recommendations and evidence levels for each clinical question have been graded according to the 2009 Oxford Centre for Evidence-Based Medicine levels of evidence and grades of recommendation. This guideline aims to provide guidance for clinical practice, improve the level of donor kidney preservation from deceased organ donation in China, reduce the rate of organ discard, and alleviate the shortage of organs.