罗德, 刘江, 周鹏程, 等. 低温机械灌注对大鼠肾脏炎症因子表达水平的影响[J]. 器官移植, 2023, 14(6): 824-830. DOI: 10.3969/j.issn.1674-7445.2023132
引用本文: 罗德, 刘江, 周鹏程, 等. 低温机械灌注对大鼠肾脏炎症因子表达水平的影响[J]. 器官移植, 2023, 14(6): 824-830. DOI: 10.3969/j.issn.1674-7445.2023132
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Luo De, Liu Jiang, Zhou Pengcheng, et al. Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney[J]. ORGAN TRANSPLANTATION, 2023, 14(6): 824-830. DOI: 10.3969/j.issn.1674-7445.2023132
Citation: Luo De, Liu Jiang, Zhou Pengcheng, et al. Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney[J]. ORGAN TRANSPLANTATION, 2023, 14(6): 824-830. DOI: 10.3969/j.issn.1674-7445.2023132
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低温机械灌注对大鼠肾脏炎症因子表达水平的影响

Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney

    摘要
  • 摘要:
      目的   探讨低温机械灌注（HMP）对大鼠肾脏炎症因子表达水平的影响。
      方法  雄性大鼠30只，随机分为对照组（Control组），静态冷保存组（SCS组）和HMP组，每组10只。记录HMP过程中流速、肾内阻力和灌注流出液pH值。收集每组肾脏组织，采用逆转录聚合酶链反应（RT-PCR）检测CXC趋化因子配体（CXCL）1、CXCL2、干扰素（IFN）-β1、IFN-α4、CC趋化因子配体（CCL）2、CCL20、白细胞介素（IL）-17α、IL-17C和肿瘤坏死因子（TNF）-α的信使RNA（mRNA）表达水平，苏木素-伊红（HE）染色观察肾脏组织病理学改变。
      结果  HMP过程中的流速、肾内阻力保持稳定，灌注流出液pH值缓慢降低。RT-PCR结果显示，与Control组比较，SCS组和HMP组CXCL1、CXCL2、CCL2、CCL20、IL-17α、IL-17C和TNF-α的mRNA相对表达量升高；与SCS组比较，HMP组CXCL1、CXCL2、CCL2、CCL20、IL-17α和TNF-α的mRNA相对表达量升高（均为P<0.05）。HE染色结果显示，Control组肾脏细胞形态正常，SCS组出现明显的上皮坏死、胞质空泡化、刷状缘丢失、上皮脱落，与SCS组比较，HMP组病理改变程度减轻。
      结论  HMP能激活肾脏炎症反应，抑制HMP过程中的炎症反应激活有望进一步提高移植物保存效果。

     

    Abstract:
      Objective  To evaluate the effect of hypothermic machine perfusion (HMP) on the expression levels of inflammatory cytokines in rat kidney.
      Methods  Thirty male rats were randomly divided into the control (Control group), static cold storage group (SCS group) and HMP group, with 10 rats in each group. The velocity, intrarenal resistance and pH value of perfusion effluent were recorded during HMP. The expression levels of CXC chemokine ligand (CXCL)1, CXCL2, interferon (IFN)-β1, IFN-α4, CC chemokine ligand (CCL)2, CCL20, interleukin (IL)-17α, IL-17C and tumor necrosis factor (TNF)-α messenger RNA (mRNA) in renal tissues were evaluated by reverse transcription polymerase chain reaction (RT-PCR). Pathological changes of the kidney were observed by hematoxylin-eosin (HE) staining.
      Results  During HMP, the velocity and intrarenal resistance remained stable, and the pH value of perfusion effluent was decreased slowly. RT-PCR showed that the relative expression levels of CXCL1, CXCL2, CCL2, CCL20, IL-17α, IL-17C and TNF-α mRNA in the SCS and HMP groups were higher compared with those in the Control group. Compared with the SCS group, the relative expression levels of CXCL1, CXCL2, CCL2, CCL20, IL-17α and TNF-α mRNA were up-regulated in the HMP group (all P<0.05). HE staining revealed that the morphology of renal cells was normal in the Control group, whereas evident epithelial necrosis, cytoplasmic vacuolation, brush border loss and epithelial shedding were observed in the SCS group. Compared with the SCS group, pathological changes in the HMP group were alleviated.
      Conclusions  HMP may activate renal inflammation, and inhibiting the activation of inflammation during HMP is expected to further improve the effect of allograft preservation.

     

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