鲍志野, 朱嘉亿, 蹇骞, 等. 建立小鼠腹部心脏移植模型联合尾静脉注射的实践体会（附视频）[J]. 器官移植, 2019, 10(2): 171-174. DOI: 10.3969/j.issn.1674-7445.2019.02.009
引用本文: 鲍志野, 朱嘉亿, 蹇骞, 等. 建立小鼠腹部心脏移植模型联合尾静脉注射的实践体会（附视频）[J]. 器官移植, 2019, 10(2): 171-174. DOI: 10.3969/j.issn.1674-7445.2019.02.009
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Bao Zhiye, Zhu Jiayi, Jian Qian, et al. Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)[J]. ORGAN TRANSPLANTATION, 2019, 10(2): 171-174. DOI: 10.3969/j.issn.1674-7445.2019.02.009
Citation: Bao Zhiye, Zhu Jiayi, Jian Qian, et al. Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)[J]. ORGAN TRANSPLANTATION, 2019, 10(2): 171-174. DOI: 10.3969/j.issn.1674-7445.2019.02.009
shu

建立小鼠腹部心脏移植模型联合尾静脉注射的实践体会（附视频）

Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)

    摘要
  • 摘要:
      目的  总结建立稳定的小鼠腹部心脏移植模型联合尾静脉注射的实践体会。
      方法  实验练习包括50对供、受体为昆明小鼠的同系移植，40对供、受体为C57BL/6J小鼠的同系移植；正式实验包括10对供、受体为C57BL/6J小鼠的同系移植，30对以Balb/c小鼠为供体、C57BL/6J小鼠为受体的同种异系移植。记录手术过程中每个步骤的时间（包括供体心脏摘取和修整时间、受体血管吻合等）。术后每日观察移植心脏搏动持续时间及受体存活时间，同时记录移植小鼠的尾静脉注射所需时间。同系移植术后30 d、同种异系移植术后7 d行移植心脏病理学检查（各5只）。
      结果  正式实验心脏移植成功率为90%。供体心脏摘取和修整时间为（13.9±0.6）min，受体冷缺血时间为（14.2±1.2）min，血管吻合时间为（34.2±3.1）min，总手术时间为（86.6±5.4）min，术后同系移植小鼠移植心脏存活时间达到100 d以上，术后30 d病理学检查显示仅有少量炎症细胞浸润。同种异系移植小鼠因发生排斥反应，存活时间为（7.2±0.5）d。术后7 d病理学检查示心肌大量炎症细胞浸润，呈急性细胞性排斥反应表现。尾静脉注射超过200次后，成功率达90%。
      结论  本研究成功建立了稳定的小鼠腹部心脏移植模型。同时进行尾静脉注射可有效地利用预实验的小鼠。

     

    Abstract:
      Objective  To summarize the practice experience of establishing a stable abdominal heart transplantation model combined with tail vein injection in mice.
      Methods  In the preliminary experiment, 50 pairs of donor and recipient Kunming mice received isotransplantation, 40 pairs of donor and recipient C57BL/6J mice underwent isotransplantation. In the formal experiment, 10 pairs of donor and recipient C57BL/6J mice received isotransplantation, 30 pairs of Balb/c mice as the donor and C57BL/6J mice as the recipient received allotransplantation. The time of each step of the heart transplantation (including harvesting and dressing of the donor heart, vascular anastomosis of the recipient, etc.) was recorded. The duration of transplanted heart beat and the survival time of the recipient was observed daily after operation. The time required for tail vein injection in the transplanted mice was recorded. Pathological examination of the transplanted heart was performed at 30 d after isotransplantation (n=5) and 7 d after allotransplantation (n=5).
      Results  In the formal experiment, the success rate of heart transplantation was 90%. The harvesting and dressing time of donor heart was (13.9±0.6) min. The cold ischemia time of the recipient was (14.2±1.2) min. The vascular anastomosis time was (34.2±3.1) min. The total operation time was (86.6±5.4) min. Postoperatively, the transplanted heart of the mice undergoing isotransplantation survived longer than 100 d. Pathological examination at postoperative 30 d demonstrated only a slight amount of inflammatory cell infiltration. The survival time of the mice receiving allotransplantation was (7.2±0.5) d due to rejection reaction. At postoperative 7 d, pathological examination showed a large quantity of inflammatory cells infiltrating into the myocardium, manifested with acute cellular rejection. The success rate reached 90% after over 200 times of tail vein injection.
      Conclusions  In this study, a stable mouse abdominal heart transplantation model is successfully established. The mouse models in the preliminary experiment can be utilized for tail vein injection.

     

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