程骁, 杨家杰, 钟金淑子. 氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响[J]. 器官移植, 2018, 9(2): 116-121. DOI: 10.3969/j.issn.1674-7445.2018.02.005
引用本文: 程骁, 杨家杰, 钟金淑子. 氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响[J]. 器官移植, 2018, 9(2): 116-121. DOI: 10.3969/j.issn.1674-7445.2018.02.005
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Cheng Xiao, Yang Jiajie, Sookjia Kim Chung. Effect of oxygen glucose deprivation-reperfusion in astrocytes overexpressing endothelin-1 on the proliferation of neural stem/ progenitor cells[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 116-121. DOI: 10.3969/j.issn.1674-7445.2018.02.005
Citation: Cheng Xiao, Yang Jiajie, Sookjia Kim Chung. Effect of oxygen glucose deprivation-reperfusion in astrocytes overexpressing endothelin-1 on the proliferation of neural stem/ progenitor cells[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 116-121. DOI: 10.3969/j.issn.1674-7445.2018.02.005
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氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响

Effect of oxygen glucose deprivation-reperfusion in astrocytes overexpressing endothelin-1 on the proliferation of neural stem/ progenitor cells

    摘要
  • 摘要:
      目的  探讨氧糖剥夺-再灌注（OGD-R）星型胶质细胞内皮素（ET）-1过表达对神经干/祖细胞（NSPCs）增殖的影响。
      方法  构建阴性对照星型胶质细胞（C6-Mock）和ET-1过表达的星形胶质细胞（C6-ET-1）OGD-R模型，并构建星型胶质细胞与NSPCs Transwell共培养体系。对星型胶质细胞及原代NSPCs进行形态观察及鉴定；将细胞分为以下4组进行共培养：C6-Mock+NSPCs组，OGD-R+C6-Mock+NSPCs组，C6-ET-1+NSPCs组，OGD-R+C6-ET-1+NSPCs组，共培养0、24、48、72 h，分析各组NSPCs神经球直径。
      结果  C6-Mock及C6-ET-1均呈现Ⅰ型星型胶质细胞的纤维状形态，神经胶质酸性纤维蛋白（GFAP）表达于这两种细胞的胞质中。原代NSPCs的巢蛋白（nestin）染色阳性。共培养后的48 h及72 h，OGD-R+C6-Mock+NSPCs组神经球的直径明显大于C6-Mock+NSPCs组的直径，OGD-R+C6-ET-1+NSPCs组神经球的直径明显大于C6-ET-1+NSPCs组的直径，同时OGD-R+C6-ET-1+NSPCs组神经球的直径明显大于OGD-R+C6-Mock+NSPCs组的直径，差异均有统计学意义（均为P < 0.05）。
      结论  OGD-R星型胶质细胞促进NSPCs的增殖，且ET-1过表达进一步促进了NSPCs的增殖。

     

    Abstract:
      Objective  To investigate the effect of oxygen glucose deprivation-reperfusion (OGD-R) in astrocytes overexpressing endothelin (ET)-1 on the proliferation of neural stem/progenitor cells (NSPCs).
      Methods  OGD-R models of negative control astrocytes (C6-Mock) and astrocytes over-expressing ET-1 (C6-ET-1) were constructed. Transwell coculture system of astrocytes and NSPCs was established. Morphologic observation and identification of the astrocytes and primary NSPCs were performed. The cells were divided into four groups: C6-Mock+NSPCs, OGD-R+C6-Mock+NSPCs, C6-ET-1+NSPCs and OGD-R+C6-ET-1+NSPCs groups and co-cultured for 0, 24, 48 and 72 h respectively. The diameter of neurosphere was measured in each group.
      Results  In the C6-Mock and C6-ET-1 cells, typeⅠastrocytes in fibrous morphology were observed. Glial fibrillary acidic protein (GFAP) was expressed in the cytoplasm of these two types of cells. Primary NSPCs were positive for nestin staining. After co-culture for 48 and 72 h, the neurosphere diameter in the OGD-R+C6-Mock+NSPCs group was significantly greater than that in the C6-Mock+NSPCs group. The neurosphere diameter in the OGD-R+C6-ET-1+NSPCs group was considerably greater than that in the C6-ET-1+NSPCs group. The neurosphere diameter in the OGD-R+C6-ET-1+NSPCs group was significantly greater compared with that in the OGD-R+C6-Mock+NSPCs group (all P < 0.05).
      Conclusions  OGD-R astrocytes can promote the proliferation of NSPCs. ET-1 over-expression further accelerates the proliferation of NSPCs.

     

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