白剑, 肖漓, 苗兰英, 等. 人类白细胞抗原-G阳性的脐带间充质干细胞 体外诱导调节性T细胞的实验研究[J]. 器官移植, 2018, 9(2): 97-102. DOI: 10.3969/j.issn.1674-7445.2018.02.002
引用本文: 白剑, 肖漓, 苗兰英, 等. 人类白细胞抗原-G阳性的脐带间充质干细胞 体外诱导调节性T细胞的实验研究[J]. 器官移植, 2018, 9(2): 97-102. DOI: 10.3969/j.issn.1674-7445.2018.02.002
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Bai Jian, Xiao Li, Miao Lanying, et al. Experimental study on the in vitro induction of regulatory T cells by umbilical cord mesenchymal stem cells with positive human leukocyte antigen-G[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 97-102. DOI: 10.3969/j.issn.1674-7445.2018.02.002
Citation: Bai Jian, Xiao Li, Miao Lanying, et al. Experimental study on the in vitro induction of regulatory T cells by umbilical cord mesenchymal stem cells with positive human leukocyte antigen-G[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 97-102. DOI: 10.3969/j.issn.1674-7445.2018.02.002
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人类白细胞抗原-G阳性的脐带间充质干细胞 体外诱导调节性T细胞的实验研究

Experimental study on the in vitro induction of regulatory T cells by umbilical cord mesenchymal stem cells with positive human leukocyte antigen-G

    摘要
  • 摘要:
      目的  探讨人类白细胞抗原（HLA）-G阳性的脐带间充质干细胞在体外诱导调节性T细胞（Treg）产生的效果。
      方法  从新生儿脐带中分离脐带间充质干细胞，采用脂质体转染的方式将PEGFP-N1-HLA-G质粒转染到脐带间充质干细胞中，设为PEGFP-N1-HLA-G组; 转染空载体PEGFP-N1质粒的脐带间充质干细胞设为PEGFP-N1组; 相同条件下，未加入空载体的脐带间充质干细胞设为空白对照组。采用流式细胞仪检测脐带间充质干细胞标志物; 采用蛋白质免疫印迹法鉴定各组细胞HLA-G蛋白的表达; 各组细胞与健康人外周血中CD4+T细胞混合培养24 h和48 h后，采用流式细胞仪检测CD4+ CD25+ Foxp3+Treg占全部T细胞的比例。
      结果  脐带间充质干细胞CD45、CD34和HLA-DR呈阴性表达，CD29、CD44和CD105呈阳性表达; PEGFP-N1-HLA-G组可以表达HLA-G蛋白，与空白对照组和PEGFP-N1组比较差异均有统计学意义（均为P < 0.01）。PEGFP-N1-HLA-G组细胞在与CD4+T细胞混合培养24 h后，CD4+ CD25+ Foxp3+Treg占全部T细胞的（15.3±1.9）%，在培养48 h后，CD4+ CD25+ Foxp3+Treg占全部T细胞的（14.3±2.1）%，与空白对照组和PEGFP-N1组比较，差异均有统计学意义（均为P < 0.05）。
      结论  HLA-G基因修饰后脐带间充质干细胞能够有效地在体外诱导CD4+ CD25+ Foxp3+Treg的产生。

     

    Abstract:
      Objective  To explore the effect of umbilical cord mesenchymal stem cells with positive human leukocyte antigen (HLA)-G on inducing the production of regulatory T cells (Treg) in vitro.
      Methods  Umbilical cord mesenchymal stem cells were isolated from umbilical cord of neonates. PEGFP-N1-HLA-G plasmid was transfected into the human umbilical cord mesenchymal stem cells by liposome transfection, as PEGFP-N1-HLA-G group. PEGFP-N1 empty vector plasmid was transfected into the human umbilical cord mesenchymal stem cells, as PEGFP-N1 group. The human umbilical cord mesenchymal stem cells without empty vector under the same conditions were set as blank control group. Markers of the umbilical cord mesenchymal stem cells were detected using flow cytometry. The expression of HLA-G protein in each group of cells was identified by Western Blot. After mixed-culturing with CD4+T cells in peripheral blood of healthy subjects for 24 h and 48 h, the proportion of CD4+ CD25+ Foxp3+Treg in total T cells of each group was detected by flow cytometry.
      Results  CD45, CD34 and HLA-DR presented negative expression on umbilical cord mesenchymal stem cells, while CD29, CD44 and CD105 presented positive expression. HLA-G protein could be expressed in the PEGFP-N1-HLA-G group, which had statistically significant difference compared with the blank control group and PEGFP-N1 group (both P < 0.01). After PEGFP-N1-HLA-G group and CD4+T cells were mixed-cultured for 24 h and 48 h, CD4+ CD25+ Foxp3+Treg accounted for (15.3±1.9)% and (14.3±2.1)% of the total T cells respectively, both of which presented statistically significant difference compared with the blank control group and PEGFP-N1 group (all P < 0.05).
      Conclusions  Umbilical cord mesenchymal stem cells with HLA-G gene modified can effectively induce the production of CD4+ CD25+ Foxp3+Treg in vitro.

     

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