王玲, 拉佈旦白拉. 允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响[J]. 器官移植, 2016, 7(5): 365-369. DOI: 10.3969/j.issn.1674-7445.2016.05.007
引用本文: 王玲, 拉佈旦白拉. 允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响[J]. 器官移植, 2016, 7(5): 365-369. DOI: 10.3969/j.issn.1674-7445.2016.05.007
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Wang Ling, LaBudanbaila. Effect of permissive hypercapnia on CD4+ and CD8+ T cells of rats with acute rejection after lung transplantation[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 365-369. DOI: 10.3969/j.issn.1674-7445.2016.05.007
Citation: Wang Ling, LaBudanbaila. Effect of permissive hypercapnia on CD4+ and CD8+ T cells of rats with acute rejection after lung transplantation[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 365-369. DOI: 10.3969/j.issn.1674-7445.2016.05.007
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允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响

Effect of permissive hypercapnia on CD4+ and CD8+ T cells of rats with acute rejection after lung transplantation

    摘要
  • 摘要:
      目的  探讨允许性高碳酸血症在大鼠肺移植急性排斥反应中对CD4+、CD8+T细胞的影响。
      方法  24只Wistar雄性大鼠和12只SD雄性大鼠配对，随机分成3组(每组6对)。对照组和治疗组分别以SD大鼠和Wistar大鼠为供、受体，同种移植组以Wistar大鼠为供、受体。采用Cuff法建立大鼠左肺原位移植急性排斥反应模型。治疗组再灌注后吸入50%的氧气和8%的二氧化碳，对照组和同种移植组再灌注后吸入50%的氧气。术后7 d采用免疫组织化学(免疫组化)方法检测移植肺组织中CD4+和CD8+T细胞的表达情况；采用流式细胞仪检测外周血中CD4+和CD8+T细胞的比例；采用酶链免疫吸附试验(ELISA)测外周血中白细胞介素(IL)-2和干扰素(IFN)-γ的水平。
      结果  免疫组化结果显示，与对照组相比，治疗组和同种移植组大鼠移植肺组织中CD8+T细胞表达均明显减少。流式细胞分析结果显示，与对照组比较，治疗组和同种移植组的CD8+T细胞比例均明显降低(均为P＜0.05)。ELISA结果显示，与对照组比较，治疗组和同种移植组的IL-2和IFN-γ水平均明显降低(均为P＜0.05)。
      结论  允许性高碳酸血症通过抑制CD8+ T细胞的增殖及CD4+ T细胞释放炎症因子，从而抑制肺移植后急性排斥反应。

     

    Abstract:
      Objective  To investigate the effect of permissive hypercapnia on CD4+ and CD8+ T cells of rats with acute rejection after lung transplantation.
      Methods  Twenty-four male Wistar rats and 12 male SD rats were paired and randomly divided into 3 groups (6 pairs per group). SD and Wistar rats were used as the donors and recipients in the control group and treatment group, respectively, and Wistar rats were used as the donors and recipients in the allograft group. Acute rejection rat model of orthotopic left lung transplantation was established by Cuff method. The treatment group was treated with 50% oxygen and 8% carbon dioxide after reperfusion, but only 50% oxygen for the control group and allograft group after reperfusion. Expressions of CD4+ and CD8+T cells in transplanted lung tissue were detected with immunohistochemical (IHC) method on 7 d after the operation. Proportion of CD4+ and CD8+T cells in peripheral blood was detected with flow cytometry. Furthermore, levels of interleukin (IL)-2 and interferon (IFN)-γ in peripheral blood were detected with enzyme-linked immune absorbent assay (ELISA).
      Results  The IHC results showed that, compared with the control group, the expression of CD8+T cells in transplant lung tissue of rats decreased significantly in both the treatment group and allograft group. The results of flow cytometry showed that compared with the control group, the proportion of CD8+T cells decreased significantly in both treatment group and allograft group (both P < 0.05). ELISA results showed that, compared with the control group, levels of IL-2 and IFN-γ decreased significantly in both treatment group and allograft group (both P < 0.05).
      Conclusions  Permissive hypercapnia can inhibit the acute rejection after lung transplantation through inhibiting the proliferation of CD8+T cells and release of inflammatory cytokines in CD4+ T cells.

     

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