Abstract:
Objective To evaluate the effect of intraportal injection of mesenchymal stem cells (MSC) combined with dopamine-2 receptor antagonist (eticlopride) upon accelerating the repairing of bile duct in rabbit models with ischemia-type biliary lesion (ITBL).
Methods Twenty-four New Zealand rabbits were randomly divided into four groups (6 in each group) including group A (sham surgery group), group B (ITBL group), group C (MSC group) and group D (MSC+ eticlopride group). The changes in the liver function parameters were monitored before surgery and 1 d, 4 d, 1-, 2-, 3-and 4-week after operation, respectively. At postoperative 4 weeks, cholangiography and ultrasound angiography were performed and liver tissue was sampled for pathological examination.
Results Compared with group A, the levels of postoperative liver function parameters in the groups B, C and D were significantly elevated to varying degree(all P < 0.05). Compared with group B, the liver function parameters in the groups C and D were considerably declined (both P < 0.05), especially the group D. The result of biliary tract ultrasound revealed that the ratio of portal vein diameter/bile duct diameter in groups A to D was (3.2±0.3):1, (1.2±0.2):1, (2.1±0.1):1 and (3.0±0.3):1, respectively. The most severe cholangiectasis was observed in group B, relatively slight cholangiectasis in group C and basically normal outcomes in group D. Ultrasound angiography suggested that the most significant improvement in blood supply of the bile duct walls was noted in group D. Pathological examination revealed that the severity of bile duct lesion was the slightest in group D.
Conclusions Intraportal injection of MSC in combination with dopamine-2 receptor antagonist can significantly accelerate the repairing of bile tract lesion in rabbit models with ITBL.
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