Abstract:
Objective To investigate the application value of ImmuKnow immune cell function assay in monitoring of immune function changes after renal transplantation.
Methods One hundred and six patients with uraemia undergoing renal transplantation in the Department of Organ Transplantation of the Second Affiliated Hospital of Guangzhou Medical University from January 2013 to December 2014 were included. Blood specimens were collected before transplantation and at the occurrence of infection or acute rejection during 12 months after transplantation. ImmoKnow was used to determine the adenosine triphosphate(ATP) content in CD4+T cells. The ATP content of patients with renal transplantation at different clinical conditions were observed and compared, including periopreative group, stable group, acute rejection group and infecticn group(including severe pneumonia). The ratio of T cell subsets (CD4+T cells, CD8+T cells) and natural killer (NK) cells in peripheral blood were detected. Pearson correlation analysis was used to detect the association between ATP and the blood trough concentration of tacrolimus (FK506) and ciclosporin (CsA).
Results The ATP content of the patients in the infection group was lower than that of the patients in the stable group (P < 0.001). The ATP content of patients with severe pneumonia was lower than that of patients with other infections (P < 0.05). The percentage of CD4+T cells of the patients in the infection group was lower than that of the patients in the postoperative stable group (P < 0.05). The ATP content was not associated with the postoperative blood trough concentration of FK506 and CsA.
Conclusions ImmuKnow assay may be used to monitor the postoperative immune function of patients after renal transplantation. The detection of ATP content in CD4+T cells has hinting and pre-warning function for postoperative infection, especially for severe pneumonia.
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