肾缺血-再灌注损伤大鼠SDF-1、ICAM-1表达与肾小管坏死评分的相关性研究

Relationship between the expression of SDF-1, ICAM-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury

    摘要
  • 摘要:
      目的   探讨肾缺血-再灌注损伤(IRI)大鼠基质细胞衍生因子(SDF)-1、细胞间黏附分子-1(ICAM-1)与肾小管坏死评分的相关性。
      方法   将60只SD大鼠随机分成手术组、假手术组两组,每组各30只。根据手术后检测时间不同,每组再分为6个不同时段的亚组(1、6、12、24、48、72 h组),每个亚组有5只大鼠。手术组建立大鼠肾IRI模型,假手术组大鼠仅予游离双侧肾动脉后缝合切口。检测各个时间点肾功能、肾小管坏死评分及肾脏组织SDF-1、ICAM-1表达变化。对手术组大鼠肾组织SDF-1、ICAM-1表达与肾功能和肾小管坏死评分进行Pearson直线相关分析。
      结果   手术组术后血尿素氮(BUN)、血清肌酐(Scr)较术前及相应时间段假手术亚组明显升高(均为P<0.05),且于术后12 h显著升高,高峰期在术后48 h。手术组肾小管坏死评分随时间的延长逐渐增高(均为P<0.05);肾小管坏死评分最高在手术48 h组(P<0.05)。与手术1 h组比较,手术6 h组大鼠肾组织SDF-1、ICAM-1表达开始明显增多(均为P<0.05);手术后48 h达高峰,于手术后72 h开始下降。手术组的肾组织SDF-1、ICAM-1表达与术后各时间段BUN、Scr、肾小管坏死评分呈正相关(r=0.614、0.662、0.751;0.640、0.703、0.785;均为P<0.05)。
      结论   当大鼠肾组织发生IRI时,SDF-1、ICAM-1表达上调,BUN、Scr升高,肾小管坏死评分升高,而且SDF-1、ICAM-1的表达与BUN、Scr、肾小管坏死评分呈正相关,提示SDF-1、ICAM-1表达增高程度可以作为反映肾IRI后严重程度的指标。

     

    Abstract:
      Objective   To investigate the relationship between the expression of stromal cell derived factor (SDF)-1, intercellular adhesion molecule (ICAM)-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury (IRI).
      Methods   Sixty Sprague-Dawley (SD) rats were randomly divided into operation group and sham operation group with 30 rats in each group. Then each group was divided into 6 subgroups (1 h,6 h,12 h,24 h, 48 h or 72 h) according to different time of measurement after operation with 5 rats in each subgroup. Renal IRI mode1s were built in rats of operation group. The bilateral renal arteries were dissected in rats of sham operation group and then the incision was sutured. The renal function, renal tubular necrosis score and the variation of SDF-1, ICAM-1 expression in renal tissues at different time points were measured. The relationship between the expression of SDF-1, ICAM-1 in renal tissues and renal tubular necrosis score in rats of operation group was analyzed by Pearson correlation analysis.
      Results   The levels of urea nitrogen (BUN) and serum creatinine (Scr) after operation in each subgroup of operation group were significantly higher than those before operation and those in the corresponding subgroups of sham operation group(all in P<0.05). They increased significantly 12 h after operation and reached the peak at 48 h after operation. The renal tubular necrosis score in operation group increased gradually over time (all in P<0.05). The highest renal tubular necrosis score was in the 48 h operation subgroup (P<0.05). Compared with those in 1 h operation subgroup, the expression of SDF-1, ICAM-1 in rats' renal tissues of 6 h operation subgroup began to increase significantly, and they reached the peak at 48 h after operation and began to drop down at 72 h after operation. The expression of SDF-1, ICAM-1 in rats renal tissues in operation group were positively correlated with the levels of BUN, Scr and renal tubular necrosis score at different time points after operation (r=0.614, 0.662, 0.751;0.640, 0.703, 0.785;P<0.05).
      Conclusion   When rat's renal tissue develops IRI, the expression of SDF-1, ICAM-1, BUN, Scr and renal tubular necrosis score increased.The expression of SDF-1, ICAM-1 are positively correlated with BUN, Scr and renal tubular necrosis score. The increased expression of SDF-1, ICAM-1 can serve as an indicator of the severity of renal IRI.

     

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