“钟摆式”大鼠腹部心脏异位移植改良模型的建立与评估

Establishment and evaluation of pendulum-like modified rat abdominal heart heterotopic transplantation model

    摘要
  • 摘要:
    目的  介绍“钟摆式”大鼠腹部心脏异位移植改良模型的建模方法，并评估模型质量。
    方法 由1名无移植经验的术者连续建模15台，记录各步骤时间、体质量变化及改良Stanford评分，计算手术成功率、术后1周存活率和技术成功率，受体于术后1周行超声检查。
    结果 供心获取、供心处理、受体准备和移植吻合时间分别为（14.3±1.4）min、（3.5±0.6） min、（13.6±2.1）min和（38.3±5.2）min。手术成功率为87%（13/15），术后1周存活率为100%（13/13）。改良Stanford评分提示技术成功率为92%（12/13），术后1周超声检查提示Stanford评分≥3分的移植物均可检测到明显的搏动和血流信号。
    结论 “钟摆式”大鼠腹部心脏异位移植改良模型进一步优化了操作步骤、成功率较高且质量稳定，未来可作为心脏移植基础研究的建模选择。

     

    Abstract:
    Objective To introduce the modeling method of pendulum-like modified rat abdominal heart heterotopic transplantation model and evaluate the quality of the model.
    Methods An operator without transplantation experience performed 15 consecutive models, recorded the time of each step, changes in body weight and modified Stanford scores, and calculated the surgical success rate, postoperative 1-week survival rate and technical success rate. Ultrasound examinations was performed in 1 week postoperatively.
    Results The times for donor heart acquisition, donor heart processing, recipient preparation and transplantation anastomosis were (14.3±1.4) min, (3.5±0.6) min, (13.6±2.1) min and (38.3±5.2) min respectively. The surgical success rate was 87% (13/15), and the survival rate 1 week after operative was 100% (13/13). The improved Stanford score indicated a technical success rate of 92% (12/13), and the postoperative 1-week ultrasound examination showed that grafts with Stanford scores ≥3 had detectable pulsation and blood flow signals.
    Conclusions The pendulum-like modified rat abdominal heart heterotopic transplantation improved model further optimizes the operational steps with a high success rate and stable quality, may be chosen as a modeling option for basic research in heart transplantation in the future.

     

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