Single cell sequencing reveals the antigen presentation characteristics of dendritic cells and B cells in cardiac grafts

Zhu Yuexing; Chen Chao; Xu Ye; Fan Yuxi; Zheng Xinguo; Luo Qiulin; Tang Zhouqi; Zhang Hedong; Li Tengfang; Peng Longkai; Dai Helong

doi:10.3969/j.issn.1674-7445.2024115

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Zhu Yuexing, Chen Chao, Xu Ye, et al. Single cell sequencing reveals the antigen presentation characteristics of dendritic cells and B cells in cardiac grafts[J]. ORGAN TRANSPLANTATION. doi: 10.3969/j.issn.1674-7445.2024115

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Single cell sequencing reveals the antigen presentation characteristics of dendritic cells and B cells in cardiac grafts

doi: 10.3969/j.issn.1674-7445.2024115

Zhu Yuexing^{1, 2
,},
Chen Chao^{1, 2},
Xu Ye^{1, 2},
Fan Yuxi^{2, 3},
Zheng Xinguo^{2, 3},
Luo Qiulin^{2, 3},
Tang Zhouqi²,
Zhang Hedong²,
Li Tengfang²,
Peng Longkai²,
Dai Helong^{2
,
,}

1.
Medical College of Guangxi University, Nanning, 530004, China

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Corresponding author: Dai Helong, Email: helong68888@163.com
Received Date: 2024-04-23
Available Online: 2024-07-08

Abstract

Abstract

Objective To investigate the antigen presentation characteristics of dendritic cells (DC) and B cells in cardiac grafts. Methods The heart of BALB/c mice was transplanted into the abdominal cavity of C57BL/6J mice. CD45⁺ cells in the heart graft were extracted and sorted by flow cytometry at postoperative 5 d, and single cell RNA sequencing was performed. Taking DC and B cell subsets in cardiac grafts as the main study cells, the changing trend, antigen presenting ability and intercellular communication with T cells after heart transplantation were analyzed by bioinformatics analysis and flow cytometry. Gene ontology (GO) function enrichment difference analysis was adopted to prove the specific function and the reliability annotation of cell subsets. Results Germinal center-like B cell (GC-L B) was the B cell subset with the largest increase in quantity during the acute rejection phase, accounting for 87%. Classical DC (cDC) 2 was the only DC subset with a significant increase in quantity during acute rejection of heart transplantation, accounting for 44% of DC subset, and it occupied the highest communication intensity with T cells after heart transplantation. Mononucleated DC (moDC) and memory B cell (MBC) were the main transmitters of T cell input signals non-transplanted hearts, whereas transformed into cDC2 and GC-L B during the acute rejection phase. Among them, MBC and GC-L B were the main sources of T cell input signals in non-transplanted hearts and heart grafts. Conclusions Compared with DC, B cells occupy a higher number and weight in the intercellular communication with T cells before and after heart transplantation, prompting that the antigen presenting activity of B cells is more active and stronger than DC in the early stage of acute rejection of heart transplantation.
- Heart transplantation,
- Antigen presentation,
- B cell,
- Dendritic cell,
- Single cell sequencing,
- Acute rejection,
- Cellular communication,
- T cell

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References(38)

References

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Single cell sequencing reveals the antigen presentation characteristics of dendritic cells and B cells in cardiac grafts

doi: 10.3969/j.issn.1674-7445.2024115

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Single cell sequencing reveals the antigen presentation characteristics of dendritic cells and B cells in cardiac grafts

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