Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)

Bao Zhiye; Zhu Jiayi; Jian Qian; Pan Qi; Liu Boqian; Zhang Jingxu; Zhao Keyi; Yi Caiyu; Liu Hao

doi:10.3969/j.issn.1674-7445.2019.02.009

Volume 10 Issue 2

Mar. 2019

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Bao Zhiye, Zhu Jiayi, Jian Qian, et al. Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)[J]. ORGAN TRANSPLANTATION, 2019, 10(2): 171-174. doi: 10.3969/j.issn.1674-7445.2019.02.009

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Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)

doi: 10.3969/j.issn.1674-7445.2019.02.009

Department of Hepatobiliary Surgery, the First Hospital of China Medical University, Key Laboratory of Organ Transplantation of Liaoning Province, Shenyang 110001, China

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Corresponding author: Liu Hao, Email:liuhaodoctor@hotmail.com
Received Date: 2018-12-24
Available Online: 2021-01-19
Publish Date: 2019-03-15

Abstract

Abstract

Objective To summarize the practice experience of establishing a stable abdominal heart transplantation model combined with tail vein injection in mice. Methods In the preliminary experiment, 50 pairs of donor and recipient Kunming mice received isotransplantation, 40 pairs of donor and recipient C57BL/6J mice underwent isotransplantation. In the formal experiment, 10 pairs of donor and recipient C57BL/6J mice received isotransplantation, 30 pairs of Balb/c mice as the donor and C57BL/6J mice as the recipient received allotransplantation. The time of each step of the heart transplantation (including harvesting and dressing of the donor heart, vascular anastomosis of the recipient, etc.) was recorded. The duration of transplanted heart beat and the survival time of the recipient was observed daily after operation. The time required for tail vein injection in the transplanted mice was recorded. Pathological examination of the transplanted heart was performed at 30 d after isotransplantation (n=5) and 7 d after allotransplantation (n=5). Results In the formal experiment, the success rate of heart transplantation was 90%. The harvesting and dressing time of donor heart was (13.9±0.6) min. The cold ischemia time of the recipient was (14.2±1.2) min. The vascular anastomosis time was (34.2±3.1) min. The total operation time was (86.6±5.4) min. Postoperatively, the transplanted heart of the mice undergoing isotransplantation survived longer than 100 d. Pathological examination at postoperative 30 d demonstrated only a slight amount of inflammatory cell infiltration. The survival time of the mice receiving allotransplantation was (7.2±0.5) d due to rejection reaction. At postoperative 7 d, pathological examination showed a large quantity of inflammatory cells infiltrating into the myocardium, manifested with acute cellular rejection. The success rate reached 90% after over 200 times of tail vein injection. Conclusions In this study, a stable mouse abdominal heart transplantation model is successfully established. The mouse models in the preliminary experiment can be utilized for tail vein injection.
- Abdominal heart transplantation,
- Heart transplantation,
- Tail vein injection,
- Mouse,
- Transplantation immunity,
- Organ transplantation,
- Rejection reaction,
- Inflammatory cell

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[1]	陈莉萍.实体器官移植术后隐球菌病的研究进展[J].器官移植, 2018, 9(1):9-15.DOI: 10.3969/j.issn.1674-7445.2018.01.002. CHEN LP. Research progress of cryptococcosis after solid organ transplantation[J]. Organ Transplant, 2018, 9(1):9-15. DOI: 10.3969/j.issn.1674-7445.2018.01.002.
[2]	周结学, 刘东, 吴家清, 等.儿童心脏死亡器官捐献供者单肾成人肾移植的疗效[J].实用医学杂志, 2017, 33(1):14-17.DOI: 10.3969/j.issn.1006-5725.2017.01.004. ZHOU JX, LIU D, WU JQ, et al. Effect of single kidney transplantation from cardiac death pediatric donors to adult recipients[J]. J Pract Med, 2017, 33(1):14-17. DOI: 10.3969/j.issn.1006-5725.2017.01.004.
[3]	李巍.小鼠动物模型在移植免疫研究中的重要地位与应用[J].器官移植, 2017, 8(3):186-189. DOI: 10.3969/j.issn.1674-7445.2017.03.002. LI W. Importance and application of mouse organ transplantation models for transplant immunology research[J]. Organ Transplant, 2017, 8(3):186-189.DOI: 10.3969/j.issn.1674-7445.2017.03.002.
[4]	PLENTER RJ, ZAMORA MR, GRAZIA TJ. Four decades of vascularized heterotopic cardiac transplantation in the mouse[J]. J Invest Surg, 2013, 26(4):223-228. DOI: 10.3109/08941939.2012.755238.
[5]	BENKE K, SAYOUR AA, MÁTYÁS C, et al. Heterotopic abdominal rat heart transplantation as a model to investigate volume dependency of myocardial remodeling[J]. Transplantation, 2017, 101(3):498-505. DOI: 10.1097/TP.0000000000001585.
[6]	林善昌, 何巍, 周绍昉, 等.同种异位不停跳心脏移植模型的建立[J].广西医科大学学报, 2008, 25(1):75-77. DOI: 10.3969/j.issn.1005-930X.2008.01.030. LIN SC, HE W, ZHOU SF, et al. Establishment of allogeneic non-stop beating heart transplantation model[J]. J Guangxi Med Univ, 2008, 25(1):75-77. DOI: 10.3969/j.issn.1005-930X.2008.01.030.
[7]	陈义发, 尹辉, 梁宾勇, 等.两种异位心脏移植小鼠模型的建立和比较[J].中国实验动物学报, 2010, 18(1):17-20. doi: 10.3969/j.issn.1005-4847.2010.01.005 CHEN YF, YIN H, LIANG BY, et al. Establishment and comparison of two mouse models of celiac and cervical heterotopic heart transplantation[J]. Acta Lab Anim Sci Sin, 2010, 18(1):17-20. doi: 10.3969/j.issn.1005-4847.2010.01.005
[8]	HASEGAWA T, VISOVATTI SH, HYMAN MC, et al. Heterotopic vascularized murine cardiac transplantation to study graft arteriopathy[J]. Nat Protoc, 2007, 2(3):471-480. doi: 10.1038/nprot.2007.48
[9]	NIIMI M. The technique for heterotopic cardiac transplantation in mice: experience of 3000 operations by one surgeon[J]. J Heart Lung Transplant, 2001, 20(10):1123-1128. doi: 10.1016/S1053-2498(01)00309-6
[10]	BAXTER MG, MURPHY KL, TAYLOR PM, et al. Chloral hydrate is not acceptable for anesthesia or euthanasia of small animals[J]. Anesthesiology, 2009, 111(1): 209-210. DOI: 10.1097/ALN.0b013e3181a8617e.
[11]	SHEPPARD AM, ZHAO DL, SALVI R. Isoflurane anesthesia suppresses distortion product otoacoustic emissions in rats[J]. J Otol, 2018, 13(2):59-64. DOI: 10.1016/j.joto.2018.03.002.
[12]	PLENTER RJ, GRAZIA TJ. Murine heterotopic heart transplant technique[J]. J Vis Exp, 2014(89). DOI: 10.3791/51511.
[13]	崔得利, 谭长军, 刘忠.连续缝合间断打结吻合动脉在小鼠心脏移植中的应用[J].复旦学报(医学版), 2016, 43(2):211-214.DOI: 10.3969/j.issn.1672-8467.2016.02.015. CUI DL, TAN CJ, LIU Z. The continuous-interrupted method for arterial anastomosis in mouse heart transplantation[J]. Fudan Univ J Med Sci, 2016, 43(2):211-214. DOI: 10.3969/j.issn.1672-8467.2016.02.015.
[14]	李轶倞, 张娜, 田枫.一种简易小鼠尾静脉注射固定法——笼盖压制法[J].中国比较医学杂志, 2016, 26(10):79-81. DOI:10.3969.j.issn.1671-7856.2016.10.015. LI YL, ZHANG N, TIAN F. A simple fixing method for tail vein injection in mice-cage lid pressing method[J]. Chin J Comp Med, 2016, 26(10):79-81. DOI:10.3969.j.issn.1671-7856.2016.10.015.

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Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)

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