Volume 9 Issue 3
May  2018
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Bao Yuanda, Yi Shuhong. High-throughput sequencing analysis of single nucleotide polymorphism loci mutation in genes associated with acute rejection in liver transplantation[J]. ORGAN TRANSPLANTATION, 2018, 9(3): 205-210. doi: 10.3969/j.issn.1674-7445.2018.03.007
Citation: Bao Yuanda, Yi Shuhong. High-throughput sequencing analysis of single nucleotide polymorphism loci mutation in genes associated with acute rejection in liver transplantation[J]. ORGAN TRANSPLANTATION, 2018, 9(3): 205-210. doi: 10.3969/j.issn.1674-7445.2018.03.007
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High-throughput sequencing analysis of single nucleotide polymorphism loci mutation in genes associated with acute rejection in liver transplantation

doi: 10.3969/j.issn.1674-7445.2018.03.007

  • Department of Hepatic Surgery, Liver Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China

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  • Corresponding author: Yi Shuhong, Email: yishuhong@163.com
  • Received Date: 2018-03-05
    Available Online: 2021-01-19
  • Publish Date: 2018-05-15
    Abstract
  •   Objective  To analyze the mutation of single nucleotide polymorphism (SNP) loci in genes most significantly associated with acute rejection after liver transplantation by high-throughput sequencing.  Methods  Peripheral blood samples were collected from 68 recipients undergoing allogeneic orthotopic liver transplantation. According to the incidence of acute rejection, all patients were divided into the acute rejection group (n=13) and non-rejection group (n=55). Through the literature review, 44 mutant SNP loci associated with acute rejection were finally identified. Using 44 SNP loci as the detection targets, high-throughput sequencing analysis was performed to detect peripheral blood samples in two groups of recipients. Bioinformatics analysis revealed the mutation rate of the SNP loci of genes related to acute rejection.  Results  The mutation rate of SNP loci of the interleukin (IL)-10 TT genotype, T allele, AA genotype and A allele in the acute rejection group was significantly higher than that in the non-rejection group. The mutation rate of the SNP loci of the cell chemokine receptor (CCR) 5 AG genotype in the acute rejection group was significantly lower than that in the non-rejection group, the mutation rate of the SNP loci of the CCR5 GG genotype in the acute rejection group was significantly higher than that in the non-rejection group. The mutation rate of the SNP loci of IL-4 CT genotype in the acute rejection group was significantly higher than that in the non-rejection group, the mutation rate of the SNP loci of IL-4 TT genotype in the acute rejection group was significantly lower than that in the non-rejection group. The mutation rate of the SNP loci of nuclear factor-kappa B inhibitor alpha(NF-κBIA) C allele in the acute rejection group was significantly higher than that in the non-rejection group. The mutation rate of the SNP loci of vitamin D receptor (VDR) CC genotype and C allele in the acute rejection group was significantly lower than that in the non-rejection group. Differences were statistically significant (all P < 0.05).  Conclusions  High-throughput sequencing analysis shows the genes associated with acute rejection after liver transplantation. Among them, the mutation rate of the SNP loci is relatively high in IL-10 TT genotype, T allele, AA genotype, A allele, CCR5 GG genotype, AG genotype, IL-4 CT genotype, TT genotype, NF-κBIA C allele, VDR CC genotype and C allele.

     

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