ntibody monitoring and graft biopsy after renal transplantation contribute to early diagnosis of antibody mediated rejection

Fu Qian; Wang Changxi; Li Jun; He Runjun; Liu Longshan; Deng Suxiong; Fei Jiguang; Qiu Jiang; Chen Guodong; Huang Gang; Chen Lizhong

doi:10.3969/j.issn.1674-7445.2016.06.004

Volume 7 Issue 6

Nov. 2016

Turn off MathJax

Article Contents

Article Navigation > ORGAN TRANSPLANTATION > 2016 > 7(6): 433-437

Fu Qian, Wang Changxi, Li Jun, et al. ntibody monitoring and graft biopsy after renal transplantation contribute to early diagnosis of antibody mediated rejection[J]. ORGAN TRANSPLANTATION, 2016, 7(6): 433-437. doi: 10.3969/j.issn.1674-7445.2016.06.004

Citation:

PDF( 0 KB)

ntibody monitoring and graft biopsy after renal transplantation contribute to early diagnosis of antibody mediated rejection

doi: 10.3969/j.issn.1674-7445.2016.06.004

Organ Transplant Center, the First Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Guangzhou 510080, China

More Information

Corresponding author: Wang Changxi. E-mail:wcx6363@163.com.
Received Date: 2016-07-31
Available Online: 2021-03-19
Publish Date: 2016-11-15

Abstract

Abstract

Objective To analyze the necessity of anti-human leukocyte antigen (HLA) antibody monitoring and graft biopsy on early diagnosis of antibody-mediated rejection (AMR). Methods Fifty-one recipients with de novo donor specific antibody (dnDSA) were screened and chosen. Donor specific antibody (DSA) and its ability to bind with C1q were evaluated. Pathological biopsy of the kidney graft was performed. The recipients diagnosed with AMR were divided into the unstable and stable kidney function groups. Type of DSA, binding ability of the complement and Banff score were statistically compared between two groups. Kaplan-Meier survival analysis of the kidney graft in the recipients from non-rejection, unstable and stable kidney function groups was performed. Results Type of HLA antibody, mean fluorescent intensity (MFI) of DSA, C1q binding ability and C4d deposition in peritubular capillary did not significantly differ between the unstable and stable groups (all P>0.05). Histomorphologically, the Banff score of microvasculitis, endarteritis, renal tubule-interstitial nephritis, transplantation glomerulopathy and renal tubular atrophy-stroma fibrosis did not significantly differ between two groups (all P>0.05). In the unstable group, the accumulated survival rate of the kidney graft was significantly lower compared with that in the stable group, which was significantly lower than that of their counterparts who were ineligible for pathological diagnosis (P=0.002). Conclusions It is necessary to perform regular anti-HLA antibody monitoring and pathological puncture examination after renal transplantation, which contributes to early detection and diagnosis of AMR.
- Renal transplantation,
- Antibody mediated rejection,
- Donor-specific antibody,
- Biopsy

FullText(HTML)

References(17)

References

[1]	Djamali A, Kaufman DB, Ellis TM, et al. Diagnosis and management of antibody-mediated rejection: current status and novel approaches[J]. Am J Transplant,2014,14(2):255-271. doi: 10.1111/ajt.12589
[2]	Halloran PF, Chang J, Famulski K, et al. Disappearance of T cell-mediated rejection despite continued antibody-mediated rejection in late kidney transplant recipients[J]. J Am Soc Nephrol, 2015, 26(7):1711-1720. doi: 10.1681/ASN.2014060588
[3]	Orandi BJ, Chow EH, Hsu A, et al. Quantifying renal allograft loss following early antibody-mediated rejection[J]. Am J Transplant,2015,15(2):489-498. doi: 10.1111/ajt.v15.2
[4]	Cooper JE, Gralla J, Klem P, et al. High dose intravenous immunoglobulin therapy for donor-specific antibodies in kidney transplant recipients with acute and chronic graft dysfunction[J]. Transplantation, 2014,97(12):1253-1259. doi: 10.1097/01.TP.0000443226.74584.03
[5]	Haas M, Sis B, Racusen LC, et al. Banff 2013 meeting report: inclusion of c4d-negative antibody-mediated rejection and antibody-associated arterial lesions[J]. Am J Transplant, 2014, 14(2):272-283. doi: 10.1111/ajt.12590
[6]	Solez K, Racusen LC. The Banff classification revisited[J]. Kidney Int,2013, 83(2):201-206. doi: 10.1038/ki.2012.395
[7]	Wiebe C, Gibson IW, Blydt-Hansen TD, et al. Evolution and clinical pathologic correlations of de novo donor-specific HLA antibody post kidney transplant[J]. Am J Transplant, 2012, 12(5): 1157-1167. doi: 10.1111/j.1600-6143.2012.04013.x
[8]	Wiebe C, Gibson IW, Blydt-Hansen TD, et al. Rates and determinants of progression to graft failure in kidney allograft recipients with de novo donor-specific antibody[J]. Am J Transplant, 2015, 15(11):2921-2930. doi: 10.1111/ajt.13347
[9]	Tambur AR, Herrera ND, Haarberg KM, et al. Assessing antibody strength: comparison of MFI, C1q, and titer information[J]. Am J Transplant, 2015, 15(9): 2421-2430. doi: 10.1111/ajt.v15.9
[10]	Yell M, Muth BL, Kaufman DB, et al. C1q binding activity of de novo donor-specific HLA antibodies in renal transplant recipients with and without antibody-mediated rejection[J]. Transplantation, 2015,99(6):1151-1155. doi: 10.1097/TP.0000000000000699
[11]	Loupy A, Vernerey D, Tinel C, et al. Subclinical rejection phenotypes at 1 year post-transplant and outcome of kidney allografts[J]. J Am Soc Nephrol, 2015, 26(7): 1721-1731. doi: 10.1681/ASN.2014040399
[12]	Morath C, Opelz G, Zeier M, et al. Clinical relevance of HLA antibody monitoring after kidney transplantation[J]. J Immunol Res, 2014: 845040. http://cn.bing.com/academic/profile?id=2095098345&encoded=0&v=paper_preview&mkt=zh-cn
[13]	傅茜，曾文涛，邓素雄, 等. 酶联免疫吸附抗原板LAT1HD与LAT1240检测HLA Ⅰ类抗体的对比分析[J]. 器官移植, 2010, 1(4):234-237. http://www.cnki.com.cn/Article/CJFDTOTAL-QGYZ201004009.htm Fu Q, Zeng WT, Deng SX, et al. Contrast analysis of using tray LAT1240 and LAT1HD based on enzyme-linked immune absorbent assay in detecting anti-HLA Ⅰ antibodies[J]. Organ Transplant, 2010, 1(4):234-237. http://www.cnki.com.cn/Article/CJFDTOTAL-QGYZ201004009.htm
[14]	王庆华，尚乐乐，唐敏英, 等. Luminex技术与ELISA方法检测抗HLA抗体在肾移植急性排斥反应中的应用[J]. 中华器官移植杂志, 2013, 34(8):455-457. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZJKX201311007911.htm Wang QH, Shang LL, Tang MY, et al. Comparison of Luminex vs.ELISA method to detect HLA antibodies in renal transplantation[J]. Chin J Organ Transplant, 2013, 34(8):455-457. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZJKX201311007911.htm
[15]	Gebel HM, Bray RA. HLA antibody detection with solid phase assays: great expectations or expectations too great?[J]. Am J Transplant, 2014, 14(9): 1964-1975. doi: 10.1111/ajt.12807
[16]	Wiebe C, Nevins TE, Robiner WN, et al. The synergistic effect of class Ⅱ HLA epitope-mismatch and nonadherence on acute rejection and graft survival[J]. Am J Transplant, 2015, 15(8):2197-2202. doi: 10.1111/ajt.v15.8
[17]	朱兰, 陈刚, 谢林, 等. 肾移植术后新生HLA-DQ抗体的临床意义[J].中华医学杂志,2014,94(42):3284-3288. http://cdmd.cnki.com.cn/Article/CDMD-10422-2009247725.htm Zhu L, Chen G, Xie L, et al. Role of de novo HLA-DQ antibodies in renal transplantation: analysis of a single center data[J]. Natl Med J China, 2014,94(42):3284-3288. http://cdmd.cnki.com.cn/Article/CDMD-10422-2009247725.htm

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(1) / Tables(1)

Get Citation

PDF

XML

Article Metrics

Article views (187) PDF downloads(39)

ntibody monitoring and graft biopsy after renal transplantation contribute to early diagnosis of antibody mediated rejection

doi: 10.3969/j.issn.1674-7445.2016.06.004

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

ntibody monitoring and graft biopsy after renal transplantation contribute to early diagnosis of antibody mediated rejection

doi: 10.3969/j.issn.1674-7445.2016.06.004

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content