Volume 7 Issue 5
Sep.  2016
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Lin Hua, Chen Jiejing, Chen Huaizhou, et al. Effect of anti-HLA antibody detected by Luminex testing on clinical prognosis of renal transplantation recipients[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 386-389. doi: 10.3969/j.issn.1674-7445.2016.05.011
Citation: Lin Hua, Chen Jiejing, Chen Huaizhou, et al. Effect of anti-HLA antibody detected by Luminex testing on clinical prognosis of renal transplantation recipients[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 386-389. doi: 10.3969/j.issn.1674-7445.2016.05.011
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Effect of anti-HLA antibody detected by Luminex testing on clinical prognosis of renal transplantation recipients

doi: 10.3969/j.issn.1674-7445.2016.05.011

  • Central Laboratory of the 181st Hospital of People's Liberation Army, Guangxi Key Laboratory of Metabolic Disease Research, Guilin 541002, China

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  • Corresponding author: Sui Weiguo, Email：linhuayanbama@126.com
  • Received Date: 2016-06-10
    Available Online: 2021-01-19
  • Publish Date: 2016-09-15
    Abstract
  •   Objective  To investigate the value of anti-human leukocyte antigen (HLA) antibody level detected by Luminex testing in predicting clinical prognosis of renal transplantation recipients.  Methods  A total of 1 105 patients scheduled to undergo renal transplantation (354 successfully undergoing renal transplantation) in the 181st Hospital of Peoples Liberation Army from June 2013 to November 2015 were selected. The serum samples were collected from 1 923 cases before and after renal transplantation. The positive rate and fluorescent intensity of anti-HLA antibody were detected by Luminex testing before and after renal transplantation. The renal function of recipients was also evaluated after renal transplantation.  Results  Prior to renal transplantation, 51.0% (546/1 071) of serum samples were positive for anti-HLA antibody, including 26.0% (279/1 071) positive for anti-HLA Ⅰ antibody, 24.9% (267/1 071) positive for anti-HLA Ⅱ antibody and 11.4% (122/1 071) positive for both anti-HLA Ⅰ and anti-HLA Ⅱ antibodies. Among 354 patients undergoing renal transplantation, 59 (17%) were positive for anti-HLA antibody after renal transplantation, including 25 (4 newly positive after surgery) positive for anti-HLA Ⅰ antibody, 15 (1 newly positive after surgery) positive for anti-HLA Ⅱ antibody and 19 (4 newly positive after surgery) positive for both anti-HLA Ⅰ and anti-HLA Ⅱ antibodies. During subsequent follow-up, 13 patients positive for anti-HLA Ⅰ antibody, 5 positive for anti-HLA Ⅱ antibody and 11 positive for both anti-HLA Ⅰ and anti-HLA Ⅱ antibodies developed transplant kidney dysfunction. All patients newly positive for anti-HLA antibody after renal transplantation presented with transplant kidney dysfunction.  Conclusions  Luminex testing can perform dynamic detection of the positive rate of anti-HLA antibody, which is important in predicting clinical prognosis of recipients after renal transplantation.

     

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    • References(17)
  • [1]
    万峰春, 刘玲玲, 林春华, 等.供者特异性抗体对肾移植患者肾功能的影响[J].中华实验外科杂志, 2014, 31(9): 2055-2056.

    Wan FC, Liu LL, Lin CH, et al. The influence of donor specific antibody on renal function after renal transplantation[J]. Chin J Exp Surg, 2014, 31(9): 2055-2056.
    [2]
    匡国杰, 武洪文, 周文强, 等.单中心两种方法检测280例群体反应性抗体的对比分析[J].中国组织工程研究, 2014, 18(5): 767-772. http://www.cnki.com.cn/Article/CJFDTOTAL-XDKF201405021.htm

    Kuang GJ, Wu HW, Zhou WQ, et al. Single-center comparative analysis of panel reactive antibodies of 280 cases detected by two methods[J]. Chin J Tissue Eng Res, 2014, 18(5): 767-772. http://www.cnki.com.cn/Article/CJFDTOTAL-XDKF201405021.htm
    [3]
    Nocera A, Cioni M, Tagliamacco A, et al. Serum complement inactivation unveiled prepregnancy donor-specific HLA antibodies leading to postpartum kidney graft loss[J]. Transpl Int, 2015, 28(5):623-625. doi: 10.1111/tri.2015.28.issue-5
    [4]
    Zhang Q, Liang LW, Gjertson DW, et al. Development of posttransplant antidonor HLA antibodies is associated with acute humoral rejection and early graft dysfunction[J]. Transplantation, 2005, 79(5): 591-598. doi: 10.1097/01.TP.0000155246.52249.AC
    [5]
    Khovanova N, Daga S, Shaikhina T, et al. Subclass analysis of donor HLA-specific IgG in antibody-incompatible renal transplantation reveals a significant association of IgG4 with rejection and graft failure[J]. Transpl Int, 2015, 28(12):1405-1415. doi: 10.1111/tri.12648
    [6]
    Delville M, Charreau B, Rabant M, et al. Pathogenesis of non-HLA antibodies in solid organ transplantation: where do we stand?[J]. Hum Immunol, 2016, DOI: 10.1016/j.humimm.2016.05.021[Epub ahead of print].
    [7]
    Kim JJ, Balasubramanian R, Michaelides G, et al. The clinical spectrum of de novo donor-specific antibodies in pediatric renal transplant recipients[J]. Am J Transplant, 2014, 14(10):2350-2358. doi: 10.1111/ajt.12859
    [8]
    Ducreux S, Guillaud O, Bosch A, et al. Monitoring efficiency of humoral rejection episode therapy in liver transplantation: any role for complement binding Luminex single antigen assays?[J]. Transpl Immunol, 2016, 35:23-28. doi: 10.1016/j.trim.2016.01.007
    [9]
    Panigrahi A, Gupta N, Siddiqui JA, et al. Post transplant development of MICA and anti-HLA antibodies is associated with acute rejection episodes and renal allograft loss[J]. Human Immunol, 2007, 68(5): 362-367. doi: 10.1016/j.humimm.2007.01.006
    [10]
    邱建新, 蔡俊超, Terasaki PI, 等.肾移植受者单抗原磁珠法检测人类白细胞抗原DP抗体[J/CD].中华细胞与干细胞杂志(电子版), 2012, 2(1):27-30. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_zhxbygxbzz201201007

    Qiu JX, Cai JC, Terasaki PI, et al. Detection of antibodies to human leucocyte antigen-DP in renal transplant recipients using single antigen beads[J/CD]. Chin J Cell Stem Cell (Electr Edit), 2012, 2(1):27-30. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_zhxbygxbzz201201007
    [11]
    Marfo K, Ajaimy M, Colovai A, et al. Pretransplant immunologic risk assessment of kidney transplant recipients with donor-specific anti-human leukocyte antigen antibodies[J]. Transplantation, 2014, 98(10):1082-1088. doi: 10.1097/TP.0000000000000191
    [12]
    Banasik M, Boratynska M, Koscielska-Kasprzak K, et al. Long-term follow-up of non-HLA and anti-HLA antibodies:incidence and importance in renal transplantation[J]． Transplant Proc, 2013, 45(4):1462-1465. doi: 10.1016/j.transproceed.2012.11.025
    [13]
    张明, 顾勇, 陆福明, 等.人类白细胞抗原抗体对移植肾患者肾功能的影响[J].中国临床医学, 2013, 20(2): 161-163. http://www.cnki.com.cn/Article/CJFDTOTAL-LCYX201302020.htm

    Zhang M, Gu Y, Lu FM, et al. Impact of HLA antibodies on renal function in patients after renal transplantation[J]. Chin J Clin Med, 2013, 20(2): 161-163. http://www.cnki.com.cn/Article/CJFDTOTAL-LCYX201302020.htm
    [14]
    刘丹, 胡娟, 刘萍, 等.肾移植受者血清抗血管内皮细胞特异性抗体的提取和纯化[J].器官移植, 2015, 6(3):146-151. http://www.organtranspl.com/browse/detail/qkid/72/id/76.html

    Liu D, Hu J, Liu P, et al. Extraction and purification of serum specific endothelial cell antibody of renal transplant recipients[J]. Organ Transplant, 2015, 6(3):146-151. http://www.organtranspl.com/browse/detail/qkid/72/id/76.html
    [15]
    Crespo M, Yelamos J, Redondo D, et al. Circulating NK-cell subsets in renal allograft recipients with anti-HLA donor-specific antibodies[J]. Am J Transplant, 2015, 15(3):806-814. doi: 10.1111/ajt.13010
    [16]
    Terasaki P, Ozawa M, Castro R. Four-year follow-up of a prospective trial of HLA and MICA antibodies on kidney graft survival[J]．Am J Transplant, 2007, 7(2): 408-415. doi: 10.1111/ajt.2007.7.issue-2
    [17]
    Matignon M, Leibler C, Moranne O, et al. Anti-HLA sensitization after kidney allograft nephrectomy: changes one year post-surgery and beneficial effect of intravenous immunoglobulin[J]. Clin Transplant, 2016, 30(6):731-740. doi: 10.1111/ctr.2016.30.issue-6
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