留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

胰岛保护和功能维护的研究进展

高宏君, 邱敏华, 陈继冰. 胰岛保护和功能维护的研究进展[J]. 器官移植, 2023, 14(2): 207-212. doi: 10.3969/j.issn.1674-7445.2023.02.005
引用本文: 高宏君, 邱敏华, 陈继冰. 胰岛保护和功能维护的研究进展[J]. 器官移植, 2023, 14(2): 207-212. doi: 10.3969/j.issn.1674-7445.2023.02.005
Gao Hongjun, Qiu Minhua, Chen Jibing. Research progress on islet protection and functional maintenance[J]. ORGAN TRANSPLANTATION, 2023, 14(2): 207-212. doi: 10.3969/j.issn.1674-7445.2023.02.005
Citation: Gao Hongjun, Qiu Minhua, Chen Jibing. Research progress on islet protection and functional maintenance[J]. ORGAN TRANSPLANTATION, 2023, 14(2): 207-212. doi: 10.3969/j.issn.1674-7445.2023.02.005

胰岛保护和功能维护的研究进展

doi: 10.3969/j.issn.1674-7445.2023.02.005
基金项目: 

广西科技基地和人才专项 桂科AD22035122

详细信息
    作者简介:
    通讯作者:

    高宏君,Email:gao4056@163.com

  • 中图分类号: R617, R587.1

Research progress on islet protection and functional maintenance

More Information
  • 摘要: 胰岛移植在糖尿病及其并发症的治疗中有很广阔的前景,但移植后功能不良、排斥反应和供体不足等是胰岛移植领域的瓶颈。优化胰腺保存方法对获取有效胰岛的数量及功能的维护有积极意义;在培养期间对胰岛进行抗排斥反应和抗凋亡处理,包括间充质干细胞(MSC)、MSC来源的外泌体、抗凋亡药物和基因修饰等可能成为临床胰岛移植胰岛保护与功能维护的重要方法;胰岛移植后抗立即经血液介导的炎症反应(IBMIR)药物的使用对于胰岛功能的保护也具有重要作用。本文围绕从胰岛制备到胰岛移植的全过程,探讨胰岛保护和功能维护相关策略,旨在为提升供体质量以弥补供体绝对数量的不足,提升胰岛移植效率提供参考。

     

  • 图  1  人羊膜间充质干细胞包裹的胰岛(免疫荧光,×200)

    注:A图为MSC用Dio绿色荧光探针标记;B图为胰岛用Dil红色荧光探针标记;C图为A图和B图的合并。

    Figure  1.  Pancreatic islets coated by human amniotic mesenchymal stem cells

  • [1] CENGIZ E, DANNE T, AHMAD T, et al. ISPAD clinical practice consensus guidelines 2022: insulin treatment in children and adolescents with diabetes[J]. Pediatr Diabetes, 2022, 23(8): 1277-1296. DOI: 10.1111/pedi.13442.
    [2] 王宇琴, 朱永星, 冉隆梅, 等. 整合医学模式下2型糖尿病个性化干预的临床效果研究[J]. 实用医学杂志, 2022, 38(18): 2346-2351. DOI: 10.3969/j.issn.1006-5725.2022.18.017.

    WANG YQ, ZHU YX, RAN LM, et al. Clinical effect of personalized intervention for patients with type 2 diabetes with holistic integrative medicine[J]. J Pract Med, 2022, 38(18): 2346-2351. DOI: 10.3969/j.issn.1006-5725.2022.18.017.
    [3] 张汀滢, 刘桃丽, 胡建国, 等. 中医禁食疗法对超重/肥胖2型糖尿病患者的临床疗效[J]. 中山大学学报(医学科学版), 2022, 43(4): 613-620. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2022.0412.

    ZHANG TY, LIU TL, HU JG, et al. Clinical effects of Chinese medicine fasting therapy in overweight/obese type 2 diabetes patients[J]. J Sun Yat-sen Univ (Med Sci), 2022, 43(4): 613-620. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2022.0412.
    [4] 傅红兴. 胰岛移植概况[J/CD]. 中华细胞与干细胞杂志(电子版), 2022, 12(4): 0-I. DOI: 10.3877/cma.j.issn.2095-1221.2022.04.010.

    FU HX. Overview of islet transplantation[J/CD]. Chin J Cell Stem Cell (Electr Edit), 2022, 12(4): 0-I. DOI: 10.3877/cma.j.issn.2095-1221.2022.04.010.
    [5] KUO CH, LI HY. Pancreatic islet transplantation in type 1 diabetes: current state and future perspectives[J]. J Diabetes Investig, 2023, 14(2): 183-185. DOI: 10.1111/jdi.13942.
    [6] BERNEY T, WASSMER CH, LEBRETON F, et al. From islet of Langerhans transplantation to the bioartificial pancreas[J]. Presse Med, 2022, 51(4): 104139. DOI: 10.1016/j.lpm.2022.104139.
    [7] DANOBEITIA JS, CHLEBECK PJ, SHOKOLENKO I, et al. Novel fusion protein targeting mitochondrial DNA improves pancreatic islet functional potency and islet transplantation outcomes[J]. Cell Transplant, 2017, 26(11): 1742-1754. DOI: 10.1177/0963689717727542.
    [8] NISHIME K, MIYAGI-SHIOHIRA C, KUWAE K, et al. Preservation of pancreas in the University of Wisconsin solution supplemented with AP39 reduces reactive oxygen species production and improves islet graft function[J]. Am J Transplant, 2021, 21(8): 2698-2708. DOI: 10.1111/ajt.16401.
    [9] LEMAIRE F, SIGRIST S, DELPY E, et al. Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas[J]. J Cell Mol Med, 2019, 23(12): 8025-8034. DOI: 10.1111/jcmm.14666.
    [10] SAKAI-YONAHA M, MIYAGI-SHIOHIRA C, KUWAE K, et al. Pancreas preservation in modified histidine-lactobionate solution is superior to that in University of Wisconsin solution for porcine islet isolation[J]. Transplantation, 2022, 106(9): 1770-1776. DOI: 10.1097/TP.0000000000003636.
    [11] KUWAE K, MIYAGI-SHIOHIRA C, HAMADA E, et al. Excellent islet yields after 18-h porcine pancreas preservation by ductal injection, pancreas preservation with MK solution, bottle purification, and islet purification using iodixanol with UW solution and iodixanol with MK solution[J]. J Clin Med, 2019, 8(10): 1561. DOI: 10.3390/jcm8101561.
    [12] OTSUKA R, MIYAGI-SHIOHIRA C, KUWAE K, et al. Pancreas preservation with a neutrophil elastase inhibitor, alvelestat, contributes to improvement of porcine islet isolation and transplantation[J]. J Clin Med, 2022, 11(15): 4290. DOI: 10.3390/jcm11154290.
    [13] NOGUCHI H, MIYAGI-SHIOHIRA C, NAKASHIMA Y, et al. A novel preservation solution containing a JNK inhibitory peptide efficiently improves islet yield for porcine islet isolation[J]. Transplantation, 2019, 103(2): 344-352. DOI: 10.1097/TP.0000000000002555.
    [14] WASSMER CH, PERRIER Q, COMBESCURE C, et al. Impact of ischemia time on islet isolation success and posttransplantation outcomes: a retrospective study of 452 pancreas isolations[J]. Am J Transplant, 2021, 21(4): 1493-1502. DOI: 10.1111/ajt.16320.
    [15] DOPPENBERG JB, LEEMKUIL M, ENGELSE MA, et al. Hypothermic oxygenated machine perfusion of the human pancreas for clinical islet isolation: a prospective feasibility study[J]. Transpl Int, 2021, 34(8): 1397-1407. DOI: 10.1111/tri.13927.
    [16] KESHTKAR S, KAVIANI M, JABBARPOUR Z, et al. Hypoxia-preconditioned Wharton's Jelly-derived mesenchymal stem cells mitigate stress-induced apoptosis and ameliorate human islet survival and function in direct contact coculture system[J]. Stem Cells Int, 2020: 8857457. DOI: 10.1155/2020/8857457.
    [17] GOU W, WANG J, SONG L, et al. Alpha-1 antitrypsin suppresses macrophage activation and promotes islet graft survival after intrahepatic islet transplantation[J]. Am J Transplant, 2021, 21(5): 1713-1724. DOI: 10.1111/ajt.16342.
    [18] KESHTKAR S, KAVIANI M, SARVESTANI FS, et al. Exosomes derived from human mesenchymal stem cells preserve mouse islet survival and insulin secretion function[J]. EXCLI J, 2020, 19: 1064-1080. DOI: 10.17179/excli2020-2451.
    [19] CHEN J, CHEN J, CHENG Y, et al. Mesenchymal stem cell-derived exosomes protect beta cells against hypoxia-induced apoptosis via miR-21 by alleviating ER stress and inhibiting p38 MAPK phosphorylation[J]. Stem Cell Res Ther, 2020, 11(1): 97. DOI: 10.1186/s13287-020-01610-0.
    [20] WEN D, PENG Y, LIU D, et al. Mesenchymal stem cell and derived exosome as small RNA carrier and immunomodulator to improve islet transplantation[J]. J Control Release, 2016, 238: 166-175. DOI: 10.1016/j.jconrel.2016.07.044.
    [21] MASUDA Y, VAZIRI ND, LI S, et al. The effect of Nrf2 pathway activation on human pancreatic islet cells[J]. PLoS One, 2015, 10(6): e0131012. DOI: 10.1371/journal.pone.0131012.
    [22] MENGER MM, NALBACH L, ROMA LP, et al. Erythropoietin exposure of isolated pancreatic islets accelerates their revascularization after transplantation[J]. Acta Diabetol, 2021, 58(12): 1637-1647. DOI: 10.1007/s00592-021-01760-4.
    [23] PRUD'HOMME GJ, GLINKA Y, HASILO C, et al. GABA protects human islet cells against the deleterious effects of immunosuppressive drugs and exerts immunoinhibitory effects alone[J]. Transplantation, 2013, 96(7): 616-623. DOI: 10.1097/TP.0b013e31829c24be.
    [24] YAMANE K, ANAZAWA T, TADA S, et al. Mitomycin C treatment improves pancreatic islet graft longevity in intraportal islet transplantation by suppressing proinflammatory response[J]. Sci Rep, 2020, 10(1): 12086. DOI: 10.1038/s41598-020-69009-8.
    [25] STAELS W, VERDONCK Y, HEREMANS Y, et al. Vegf-A mRNA transfection as a novel approach to improve mouse and human islet graft revascularisation[J]. Diabetologia, 2018, 61(8): 1804-1810. DOI: 10.1007/s00125-018-4646-7.
    [26] HU M, HAWTHORNE WJ, YI S, et al. Cellular immune responses in islet xenograft rejection[J]. Front Immunol, 2022, 13: 893985. DOI: 10.3389/fimmu.2022.893985.
    [27] MOU L, SHI G, COOPER DKC, et al. Current topics of relevance to the xenotransplantation of free pig islets[J]. Front Immunol, 2022, 13: 854883. DOI: 10.3389/fimmu.2022.854883.
    [28] HAWTHORNE WJ, SALVARIS EJ, CHEW YV, et al. Xenotransplantation of genetically modified neonatal pig islets cures diabetes in baboons[J]. Front Immunol, 2022, 13: 898948. DOI: 10.3389/fimmu.2022.898948.
    [29] YAO M, DOMOGATSKAYA A, ÅGREN N, et al. Cibinetide protects isolated human islets in a stressful environment and improves engraftment in the perspective of intra portal islet transplantation[J]. Cell Transplant, 2021, 30: 9636897211039739. DOI: 10.1177/09636897211039739.
    [30] LEE CA, DHAWAN A, SMITH RA, et al. Instant blood-mediated inflammatory reaction in hepatocyte transplantation: current status and future perspectives[J]. Cell Transplant, 2016, 25(7): 1227-1236. DOI: 10.3727/096368916X691286.
    [31] YAN LL, YE LP, CHEN YH, et al. The influence of microenvironment on survival of intraportal transplanted islets[J]. Front Immunol, 2022, 13: 849580. DOI: 10.3389/fimmu.2022.849580.
    [32] LIUWANTARA D, CHEW YV, FAVALORO EJ, et al. Characterizing the mechanistic pathways of the instant blood-mediated inflammatory reaction in xenogeneic neonatal islet cell transplantation[J]. Transplant Direct, 2016, 2(6): e77. DOI: 10.1097/TXD.0000000000000590.
    [33] MOLL G, ANKRUM JA, KAMHIEH-MILZ J, et al. Intravascular mesenchymal stromal/stem cell therapy product diversification: time for new clinical guidelines[J]. Trends Mol Med, 2019, 25(2): 149-163. DOI: 10.1016/j.molmed.2018.12.006.
    [34] LEI J, ZHANG A, DENG H, et al. Intrapleural transplantation of allogeneic pancreatic islets achieves glycemic control in a diabetic non-human primate[J]. Am J Transplant, 2022, 22(3): 966-972. DOI: 10.1111/ajt.16875.
    [35] VON ZUR-MÜHLEN B, LUNDGREN T, BAYMAN L, et al. Open randomized multicenter study to evaluate safety and efficacy of low molecular weight sulfated dextran in islet transplantation[J]. Transplantation, 2019, 103(3): 630-637. DOI: 10.1097/TP.0000000000002425.
  • 加载中
图(2)
计量
  • 文章访问数:  259
  • HTML全文浏览量:  86
  • PDF下载量:  72
  • 被引次数: 0
出版历程
  • 收稿日期:  2022-12-09
  • 网络出版日期:  2023-03-15
  • 刊出日期:  2023-03-15

目录

    /

    返回文章
    返回