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骨髓间充质干细胞对小鼠缺血-再灌注急性肾损伤中IL-10和TNF-α表达的影响

蔺晨雨 陈文 马锡慧 孔祥瑞 樊文梅 韩永 肖漓 石炳毅

蔺晨雨, 陈文, 马锡慧, 等. 骨髓间充质干细胞对小鼠缺血-再灌注急性肾损伤中IL-10和TNF-α表达的影响[J]. 器官移植, 2021, 12(5): 563-570. doi: 10.3969/j.issn.1674-7445.2021.05.010
引用本文: 蔺晨雨, 陈文, 马锡慧, 等. 骨髓间充质干细胞对小鼠缺血-再灌注急性肾损伤中IL-10和TNF-α表达的影响[J]. 器官移植, 2021, 12(5): 563-570. doi: 10.3969/j.issn.1674-7445.2021.05.010
Lin Chenyu, Chen Wen, Ma Xihui, et al. Effect of bone marrow mesenchymal stem cell on the expression of IL-10 and TNF-α in mice with ischemia-reperfusion acute kidney injury[J]. ORGAN TRANSPLANTATION, 2021, 12(5): 563-570. doi: 10.3969/j.issn.1674-7445.2021.05.010
Citation: Lin Chenyu, Chen Wen, Ma Xihui, et al. Effect of bone marrow mesenchymal stem cell on the expression of IL-10 and TNF-α in mice with ischemia-reperfusion acute kidney injury[J]. ORGAN TRANSPLANTATION, 2021, 12(5): 563-570. doi: 10.3969/j.issn.1674-7445.2021.05.010

骨髓间充质干细胞对小鼠缺血-再灌注急性肾损伤中IL-10和TNF-α表达的影响

doi: 10.3969/j.issn.1674-7445.2021.05.010
基金项目: 

军队医学科技青年培育计划 18QNP035

详细信息
    作者简介:

    蔺晨雨,女,1992年生,硕士研究生,研究方向为移植免疫学,Email:lcylinchenyu@163.com

    通讯作者:

    肖漓,女,1972年生,博士,主任技师,硕士研究生导师,研究方向为移植免疫学、感染免疫学和免疫学技术,E-mail: xiaolilab309@163.com

  • 中图分类号: R617, R364.5

Effect of bone marrow mesenchymal stem cell on the expression of IL-10 and TNF-α in mice with ischemia-reperfusion acute kidney injury

More Information
  • 摘要:   目的  探讨骨髓间充质干细胞(BMSC)对小鼠缺血-再灌注急性肾损伤(IR-AKI)过程中白细胞介素(IL)-10和肿瘤坏死因子(TNF)-α表达的影响。  方法  将小鼠随机分为假手术组(对照组)、缺血-再灌注损伤组(IRI组)和BMSC治疗组(BMSC组),每组6只。检测各组小鼠肾功能及病理学改变;检测各组小鼠肾组织细胞凋亡情况;检测各组小鼠血清IL-10和TNF-α的表达水平。将小鼠BMSC随机分为对照组和缺氧复氧组(IRI组),检测各组细胞上清IL-10和TNF-α的表达水平。  结果  对照组小鼠肾组织结构正常,IRI组肾组织结构损伤严重,BMSC组损伤较轻。与对照组比较,IRI组和BMSC组肾组织损伤评分较高;与IRI组比较,BMSC组小鼠肾组织损伤学评分较低(均为P < 0.05)。与对照组比较,IRI组小鼠血清肌酐(Scr)和血尿素氮(BUN)水平升高,BMSC组BUN水平升高;与IRI组比较,BMSC组小鼠Scr和BUN水平下降(均为P < 0.05)。IRI组肾组织凋亡细胞数量多于BMSC组和对照组,BMSC组凋亡细胞数量多于对照组(均为P < 0.05)。与对照组比较,IRI组小鼠血清IL-10和TNF-α水平均升高,BMSC组小鼠血清TNF-α水平下降,IL-10水平升高;与IRI组比较,BMSC组小鼠血清IL-10和TNF-α水平均下降(均为P < 0.05)。IRI组细胞上清IL-10、TNF-α水平与对照组比较,差异均无统计学意义(P=0.080、0.627)。  结论  BMSC输注可降低肾IRI及炎症反应,其机制可能是通过抑制TNF-α表达,而非促进IL-10的表达。

     

  • 图  1  各组小鼠肾组织病理学和肾功能变化

    注:A图为各组小鼠肾组织切片的病理学图片,黑色箭头表示肾小管扩张、坏死以及炎细胞浸润(HE,×200);B图为各组小鼠肾组织损伤评分;C图为IRI 72 h后各组小鼠Scr水平;D图为IRI 72 h后各组小鼠BUN水平;与对照组比较,aP < 0.05,与IRI组比较,bP < 0.05。

    Figure  1.  Changes in renal histopathology and renal function of mice in each group

    图  2  各组小鼠肾组织细胞凋亡情况(TUNEL,×400)

    Figure  2.  Cell apoptosis of renal tissues of mice in each group

    图  3  各组小鼠外周血IL-10和TNF-α的表达情况

    注:A图为各组小鼠外周血血清中IL-10的表达情况;B图为各组小鼠外周血血清中TNF-α的表达情况;与对照组比较,aP < 0.05,与IRI组比较,bP < 0.05。

    Figure  3.  The expression of IL-10 and TNF-α in peripheral blood of mice in each group

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出版历程
  • 收稿日期:  2021-06-22
  • 网络出版日期:  2021-09-15
  • 刊出日期:  2021-09-15

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