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肾移植术后稳定状态受者淋巴细胞亚群的动态变化及其与肾功能的相关性分析

马锡慧 韩永 李彬钰 孔祥瑞 孙玉洁 肖漓

马锡慧, 韩永, 李彬钰, 等. 肾移植术后稳定状态受者淋巴细胞亚群的动态变化及其与肾功能的相关性分析[J]. 器官移植, 2020, 11(5): 559-565. doi: 10.3969/j.issn.1674-7445.2020.05.005
引用本文: 马锡慧, 韩永, 李彬钰, 等. 肾移植术后稳定状态受者淋巴细胞亚群的动态变化及其与肾功能的相关性分析[J]. 器官移植, 2020, 11(5): 559-565. doi: 10.3969/j.issn.1674-7445.2020.05.005
Ma Xihui, Han Yong, Li Binyu, et al. Dynamic changes of lymphocyte subsets and their correlation with renal function in recipients with stable graft status after renal transplantation[J]. ORGAN TRANSPLANTATION, 2020, 11(5): 559-565. doi: 10.3969/j.issn.1674-7445.2020.05.005
Citation: Ma Xihui, Han Yong, Li Binyu, et al. Dynamic changes of lymphocyte subsets and their correlation with renal function in recipients with stable graft status after renal transplantation[J]. ORGAN TRANSPLANTATION, 2020, 11(5): 559-565. doi: 10.3969/j.issn.1674-7445.2020.05.005

肾移植术后稳定状态受者淋巴细胞亚群的动态变化及其与肾功能的相关性分析

doi: 10.3969/j.issn.1674-7445.2020.05.005
基金项目: 

国家自然科学基金 81571555

详细信息
    作者简介:

    马锡慧, 女, 1985年生, 硕士, 主管技师, 研究方向为移植免疫与免疫耐受, Email:maxihui@sina.com

    通讯作者:

    肖漓, 女, 1972年生, 博士, 主任技师, 研究方向为移植免疫与免疫耐受, Email:xiaolilab309@163.com

  • 中图分类号: R617, R392.4

Dynamic changes of lymphocyte subsets and their correlation with renal function in recipients with stable graft status after renal transplantation

More Information
  • 摘要:   目的  探讨肾移植术后稳定状态受者外周血淋巴细胞亚群的动态变化及其与肾功能的相关性。  方法  筛选行首次肾移植且术后半年内移植肾功能稳定的受者45例, 采用流式细胞术(FCM)检测受者术后15 d及1、3、6个月共计180份外周血样本淋巴细胞亚群比例和绝对值。分析淋巴细胞亚群随术后时间延长的动态变化及其与血清肌酐(Scr)和血尿素氮(BUN)的相关性。  结果  受者术后4个时间点Scr值比较, 差异均无统计学意义(均为P > 0.05)。术后15 d与术后1个月、术后1个月与术后3个月BUN比较, 差异均有统计学意义(P=0.002、P=0.001);术后15 d与术后1个月比较, CD3+CD8+T细胞、CD3+CD4+T细胞、自然杀伤(NK)细胞比例及CD4/CD8比值, 差异均有统计学意义(P=0.009、P=0.004、P < 0.001、P=0.004)。B细胞比例术后15 d与术后1个月比较、术后1个月与术后3个月比较, 差异均有统计学意义(均为P < 0.001)。CD3+T细胞、CD3+CD8+T细胞、CD3+CD4+T细胞和NK细胞绝对值术后15 d与术后1个月比较, 差异均有统计学意义(P=0.001、P=0.002、P=0.003、P < 0.001)。CD3+CD8+T细胞绝对值术后3个月和术后6个月比较, 差异有统计学意义(P=0.015)。B细胞绝对值术后1个月与术后3个月比较, 差异有统计学意义(P=0.001)。淋巴细胞亚群比例和绝对值与Scr均不相关(均为P > 0.05), CD3+CD8+T细胞、NK细胞比例和绝对值与BUN均呈负相关(P < 0.001~0.05), CD3+CD4+T细胞、B细胞比例与BUN均呈正相关(P < 0.001~0.05), CD3+T细胞绝对值与BUN呈负相关(P < 0.05)。  结论  肾移植术后稳定状态受者的淋巴细胞亚群中T细胞和NK细胞术后1个月内升高至稳定状态, B细胞术后3个月内降低至稳定状态, 且淋巴细胞亚群的动态变化与BUN相关。

     

  • 图  1  肾移植受者淋巴细胞亚群比例与BUN的相关性

    Figure  1.  Correlation between the proportion of lymphocyte subsets and BUN in renal transplant recipients

    图  2  肾移植受者淋巴细胞亚群绝对值与BUN的相关性

    Figure  2.  Correlation between the absolute value of lymphocyte subsets and BUN in renal transplant recipients

    表  1  肾移植受者不同时间点的淋巴细胞亚群比例和CD4/CD8比值

    Table  1.   The proportion of lymphocyte subsets and CD4/CD8 ratio in renal transplant recipients at different time points(x±s)

    术后时间 n CD3+T细胞(%) CD3+CD8+ T细胞(%) CD3+CD4+ T细胞(%) CD4/CD8 NK细胞(%) B细胞(%)
    15 d 45 75±8 27±9 46±10 2.0±1.1 7±5 16±7
    1个月 45 76±9 31±11a 43±11a 1.6±1.0a 12±7b 10±6b
    3个月 45 77±9 32±9 43±11 1.5±0.8 13±8 8±4c
    6个月 45 79±9 33±10 42±10 1.4±0.7 14±8 7±4
    注:术后15 d与术后1个月比较, aP < 0.01、bP < 0.001;术后1个月与术后3个月比较, cP < 0.001。
    下载: 导出CSV

    表  2  肾移植受者不同时间点的淋巴细胞亚群绝对值

    Table  2.   Absolute value of lymphocyte subsets in renal transplant recipients at different time points (x±s, ×106/L)

    术后时间 n CD3+T细胞 CD3+CD8+T细胞 CD3+CD4+T细胞 NK细胞 B细胞
    15 d 45 1 243±665 452±277 752±432 100±74 258±173
    1个月 45 1 729±1 005b 744±634a 945±523a 248±179b 233±173
    3个月 45 1 699±772 723±464 932±422 298±240 166±107c
    6个月 45 1 910±819 836±469d 1 003±466 328±251 165±108
    注:术后15 d与术后1个月比较, aP < 0.01、bP < 0.001;术后1个月与术后3个月比较, cP < 0.001;术后3个月与术后6个月比较, dP < 0.05/3。
    下载: 导出CSV

    表  3  肾移植受者淋巴细胞亚群与BUN的相关性

    Table  3.   Correlation between lymphocyte subsets and BUN in renal transplant recipients

    指标 比例 绝对值
    r P r P
    CD3+T细胞与BUN -0.071 > 0.05 -0.169 < 0.05
    CD3+CD8+T细胞与BUN -0.175 < 0.05 -0.202 < 0.01
    CD3+CD4+T细胞与BUN 0.151 < 0.05 -0.090 > 0.05
    NK细胞与BUN -0.322 < 0.001 -0.316 < 0.001
    B细胞与BUN 0.423 < 0.001 0.094 > 0.05
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-05-30
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2020-09-15

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