树突状细胞分类及调节性树突状细胞获取方式的研究进展

孙彬; 魏玉香; 石炳毅

doi:10.3969/j.issn.1674-7445.2019.02.014

树突状细胞分类及调节性树突状细胞获取方式的研究进展

doi: 10.3969/j.issn.1674-7445.2019.02.014

100071 北京，中国人民解放军军事科学院军事医学研究院(孙彬)；中国人民解放军总医院第八医学中心(孙彬、魏玉香、石炳毅)

基金项目:

国家自然科学基金 81370578

详细信息

作者简介:
孙彬，男，1981年生，硕士，主治医师，研究方向为医学检验、免疫学，Email: sunbin309mh@163.com

通讯作者:
石炳毅，男，1951年生，硕士，主任医师，研究方向为器官移植，Email：shibingyi666@126.com

中图分类号: R392, R789
计量
- 文章访问数: 542
- HTML全文浏览量: 262
- PDF下载量: 49
- 被引次数: 0
出版历程
- 收稿日期: 2018-12-25
- 网络出版日期: 2021-01-19
- 刊出日期: 2019-03-15

Research progress on the classification of dendritic cell and the acquisition methods of regulatory dendritic cell

摘要

摘要: 树突状细胞(DC)虽然数量少，但其在机体免疫系统中发挥的作用却逐渐受到认可和重视。研究表明DC由不同的亚群组成，具有不同的处理抗原的能力和激活不同效应淋巴细胞等能力。其中，调节性树突状细胞(DCreg)是一群具有负向免疫调控功能的DC亚群。DCreg能够通过不同的机制诱导机体免疫耐受，在器官移植、自身免疫性疾病、肿瘤等不同领域均发挥着重要作用。但DC在体内分布广泛，且含量少，生理情况下半衰期短，建立成熟的DC培养技术，特别是如何获取足量的DCreg，是目前免疫学界研究的重要领域。本文就DC的分类和DCreg体外获取方式进行综述。
- 树突状细胞 /
- 调节性树突状细胞 /
- 细胞因子 /
- 间充质干细胞 /
- 免疫抑制剂 /
- 凋亡 /
- 基因工程技术

HTML全文

参考文献(24)

[1]	朱婷, 赵西太, 聂青和, 等.CLEC4M介导HCV负载后树突状细胞的功能变化[J].临床肝胆病杂志, 2017, 33(6):1101-1105.DOI: 10.3969/j.issn.1001-5256.2017.06.017. ZHU T, ZHAO XT, NIE QH, et al.Changes in the function of dendritic cells after HCV loading mediated by CLEC4M[J]. J Clin Hepatol, 2017, 33(6):1101-1105.DOI: 10.3969/j.issn.1001-5256.2017.06.017.
[2]	COLLIN M, BIGLEY V.Human dendritic cell subsets:an update[J]. Immunology, 2018, 154(1):3-20.DOI: 10.1111/imm.12888.
[3]	刘袁媛, 范华骅, 任亚娜, 等.小鼠调节性树突状细胞分泌的外泌体诱导免疫耐受的实验研究[J].中国实验血液学杂志, 2008, 16(2):406-410.DOI10.3969/j.issn.1009-2137.2008.02.036. doi: 10.3969/j.issn.1009-2137.2008.02.036 LIU YY, FAN HH, REN YN, et al.Immune tolerance induced by exosomes derived from regulatory dendritic cells of mice[J]. J Exp Hematol, 2008, 16(2):406-410.DOI: 10.3969/j.issn.1009-2137.2008.02.036.
[4]	李梦媛, 燕正强, 韦荣飞, 等.IL-37b通过抑制树突状细胞相关的免疫应答缓解感染性休克[J].中国比较医学杂志, 2017, 27(11):44-49. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgsydwxzz201711009 LI MY, YAN ZQ, WEI RF, et al.IL-37b alleviates septic shock through inhibition of immune responses related to dendritic cells[J]. Chin J Compar Med, 2017, 27(11):44-49. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgsydwxzz201711009
[5]	JIA L, LU J, ZHOU Y, et al.Tolerogenic dendritic cells induced the enrichment of CD4+Foxp3+regulatory T cells via TGF-β in mesenteric lymph nodes of murine LPS-induced tolerance model[J]. Clin Immunol, 2018, 197:118-129.DOI: 10.1016/j.clim.2018.09.010.
[6]	YANG R, GAO N, CHANG Q, et al.The role of IDO, IL-10, and TGF-β in the HCV-associated chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma[J]. J Med Virol, 2019, 91(2):265-271.DOI: 10.1002/jmv.25083.
[7]	ZHANG M, TANG H, GUO Z, et al.Splenic stroma drives mature dendritic cells to differentiate into regulatory dendritic cells[J]. Nat Immunol, 2004, 5(11):1124-1133.DOI: 10.1038/ni1130.
[8]	LAUSTSEN A, BAK RO, KRAPP C, et al.Interferon priming is essential for human CD34⁺cell-derived plasmacytoid dendritic cellmaturation and function[J]. Nat Commun, 2018, 9(1):3525.DOI: 10.1038/s41467-018-05816-y.
[9]	ZHANG B, LIU R, SHI D, et al.Mesenchymal stem cells induce mature dendritic cells into a novel Jagged-2-dependent regulatory dendritic cell population[J]. Blood, 2009, 113(1):46-57.DOI: 10.1182/blood-2008-04-154138.
[10]	吴萍.CD8α ⁺Jagged2^high CD11b^high调节性树突状细胞防治GVHD的实验研究[D].广州: 南方医科大学, 2013. http://cdmd.cnki.com.cn/Article/CDMD-90023-1014100041.htm
[11]	SVENSSON M, MAROOF A, ATO M, et al.Stromal cells direct local differentiation of regulatory dendritic cells[J]. Immunity, 2004, 21(6):805-816.DOI: 10.1016/j.immuni.2004.10.012.
[12]	TANG H, GUO Z, ZHANG M, et al.Endothelial stroma programs hematopoietic stem cells to differentiate into regulatory dendritic cells through IL-10[J]. Blood, 2006, 108(4):1189-1197.DOI: 10.1182/blood-2006-01-007187.
[13]	WONG KA, RODRIGUEZ A.Plasmodium infection and endotoxic shock induce the expansion of regulatory dendritic cells[J]. Immunol, 2008, 180(2):716-726. doi: 10.4049/jimmunol.180.2.716
[14]	王小茜.CD11^cintCD45RB^hiDCreg及一群新型IL-10分泌型巨噬细胞的体内外鉴定[D].呼和浩特: 内蒙古医科大学, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10132-1014012509.htm
[15]	李颖曦, 陈丹, 王小东, 等.丹柴合剂对树突状细胞的诱导分化作用[J].天津医药, 2016, 44(3):318-321. http://d.old.wanfangdata.com.cn/Periodical/tjyy201603018 LI YX, CHEN D, WANG XD, et al.The differential effects of traditional Chinese medicine Danchaiheji on dendritic cells[J]. Tianjin Med J, 2016, 44(3):318-321. http://d.old.wanfangdata.com.cn/Periodical/tjyy201603018
[16]	宋少华, 沈筱芸, 丁国善, 等.落新妇苷对小鼠骨髓来源树突状细胞成熟及免疫功能的抑制作用[J].中西医结合学报, 2010, 8(2):145-151.DOI: 10.3736/jcim20100209. SONG SH, SHEN XY, DING GS, et al.Effects of astilbin on maturation and immunologic function of mouse bone marrow-derived dendritic cells[J]. J Integr Med, 2010, 8(2):145-151.DOI: 10.3736/jcim20100209.
[17]	杨晓勇, 姚庆春, 王玮, 等.树突状细胞共刺激分子表达与免疫抑制剂的干预[J].中国组织工程研究, 2015, 19(24):3876-3881.DOI: 10.3969/j.issn.2095-4344.2015.24.020. YANG XY, YAO QC, WANG W, et al.Dendritic cell costimulatory molecule expression and immunosuppressant intervention[J]. Chin J Tissue Eng Res, 2015, 19(24):3876-3881.DOI: 10.3969/j.issn.2095-4344.2015.24.020.
[18]	ROSA FF, PIRES CF, KUROCHKIN I, et al.Direct reprogramming of fibroblasts into antigen-presenting dendritic cells[J]. Sci Immunol, 2018, 3(30):eaau4292.DOI: 10.1126/sciimmunol.aau4292.
[19]	MCMAHON G, WEIR MR, LI XC, et al.The evolving role of mTOR inhibition in transplantation tolerance[J]. J Am Soc Nephrol, 2011, 22(3):408-415.DOI: 10.1681/ASN.2010040351.
[20]	许春杰, 顾磊, 蒋春晖, 等.1, 25(OH)2D3抑制肝星状细胞激活和调控Th细胞分化治疗肝纤维化[J].实用医学杂志, 2017, 33(13):2100-2104.DOI: 10.3969/j.issn.1006-5725.2017.13.008. XU CJ, GU L, JIANG CH, et al.Treatment effect of 1, 25(OH)2D3 on liver fibrosis by inhibiting HSC activation and regulating Th cell differentiation[J]. J Pract Med, 2017, 33(13):2100-2104.DOI: 10.3969/j.issn.1006-5725.2017.13.008.
[21]	仲凯励.凋亡细胞对树突状细胞免疫功能的影响及其机制研究[D].北京: 军事医学科学院, 2011.
[22]	周浩.耐受性树突状细胞的产生机制及其诱导移植免疫耐受作用的实验研究[D].武汉: 华中科技大学, 2007. http://cdmd.cnki.com.cn/Article/CDMD-10487-2009034043.htm
[23]	黄元寿, 汪华林, 刘长波, 等.负载CTLA4Ig重组腺病毒的未成熟树突状细胞对大鼠Th细胞增殖的影响[J].现代临床医学生物工程学杂志, 2007, 13(2):69-73.DOI: 10.3760/cma.j.issn.1674-1927.2007.02.002. HUANG YS, WANG HL, LIU CB, et al.Influence of immature dendritic cells loading recombinant adenoviral CTLA4Ig on Th cell proliferation[J]. Chin J Biomed Eng, 2007, 13(2):69-73.DOI: 10.3760/cma.j.issn.1674-1927.2007.02.002.
[24]	BONHAM CA, PENG L, LIANG X, et al.Marked prolongation of cardiac allograft survival by dendritic cells genetically engineered with NF-kappa B oligodeoxyribonucleotide decoys and adenoviral vectors encoding CTLA4-Ig[J]. J Immunol, 2002, 169(6):3382-3391. doi: 10.4049/jimmunol.169.6.3382