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体外光化学疗法对皮肤移植小鼠产生调节性T细胞的影响

魏玉香 杨绍臻 肖漓 蔡明 钱叶勇 于涛 石炳毅

魏玉香, 杨绍臻, 肖漓, 等. 体外光化学疗法对皮肤移植小鼠产生调节性T细胞的影响[J]. 器官移植, 2016, 7(5): 335-359. doi: 10.3969/j.issn.1674-7445.2016.05.00
引用本文: 魏玉香, 杨绍臻, 肖漓, 等. 体外光化学疗法对皮肤移植小鼠产生调节性T细胞的影响[J]. 器官移植, 2016, 7(5): 335-359. doi: 10.3969/j.issn.1674-7445.2016.05.00
Wei Yuxiang, Yang Shaozhen, Xiao Li, et al. Effect of extracorporeal photochemotherapy on the regulatory T cells in mice with skin allograft[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 335-359. doi: 10.3969/j.issn.1674-7445.2016.05.00
Citation: Wei Yuxiang, Yang Shaozhen, Xiao Li, et al. Effect of extracorporeal photochemotherapy on the regulatory T cells in mice with skin allograft[J]. ORGAN TRANSPLANTATION, 2016, 7(5): 335-359. doi: 10.3969/j.issn.1674-7445.2016.05.00

体外光化学疗法对皮肤移植小鼠产生调节性T细胞的影响

doi: 10.3969/j.issn.1674-7445.2016.05.00
基金项目: 

国家自然科学基金 81370578

详细信息
    通讯作者:

    石炳毅,Email:shibingyi@medmail.com.cn

  • 中图分类号: R617, R392.4

Effect of extracorporeal photochemotherapy on the regulatory T cells in mice with skin allograft

More Information
  • 摘要:   目的  探讨输注体外光化学法处理的脾淋巴细胞对皮肤移植受体小鼠产生调节性T细胞(Treg)及移植物存活时间的影响。  方法  以C57BL/6小鼠为供体,BALB/c小鼠为受体,建立小鼠皮肤移植模型。分离C57BL/6和BALB/c小鼠脾淋巴细胞(CSP、BSP),制备经8-甲氧基补骨脂素联合长波紫外线(PUVA)处理的小鼠脾淋巴细胞(PUVA-SP)。根据受者静脉输注的成分将实验动物随机分为5组(每组12只):PUVA-BSP组、PUVA-CSP组、BSP组、CSP组及磷酸盐缓冲液(PBS)对照组,每组受体分别于术前7 d、手术当日和术后7 d按组别从尾静脉注入PUVA-BSP、PUVA-CSP、BSP、CSP或PBS。观察受体移植物的存活时间,检测受体外周血中CD4+CD25+Foxp3+Treg表达情况。  结果  皮肤移植术后,PUVA-BSP组和PUVA-CSP组的受体小鼠外周细胞CD4+CD25+Foxp3+Treg的比例明显高于输注BSP组、CSP组和PBS对照组;PUVA-CSP组高于PUVA-BSP,BSP组和CSP组低于PBS对照组。PUVA-BSP组和PUVA-CSP组的受者小鼠移植皮片存活时间明显长于BSP组、CSP组和PBS对照组(均为P<0.05)。  结论  输注足够数量的PUVA-SP可诱导受体体内产生较多的CD4+CD25+Foxp3+Treg,可以显著延长移植物的存活时间。

     

  • 图  1  PUVA诱导小鼠脾淋巴细胞发生凋亡的百分比

    注:A图为C57BL/6小鼠,B图为BALB/c小鼠

    Figure  1.  Apoptosis rates of splenic lymphocyte of mice induced by PUVA

    图  2  各组小鼠外周血CD4+CD25+Foxp3+Treg比例

    Figure  2.  The rates of CD4+CD25+Foxp3+ Treg of mice in each group

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出版历程
  • 收稿日期:  2016-06-10
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2016-09-15

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