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Multi-Latex多聚粒技术联合检测尿微量蛋白在肾移植术后无创诊断中的应用价值

李圣冰 陈文 李修彬 马锡慧 孙玉洁 毕丽丽 何秀云 韩永 肖漓 石炳毅

李圣冰, 陈文, 李修彬, 等. Multi-Latex多聚粒技术联合检测尿微量蛋白在肾移植术后无创诊断中的应用价值[J]. 器官移植, 2020, 11(4): 443-448. doi: 10.3969/j.issn.1674-7445.2020.04.003
引用本文: 李圣冰, 陈文, 李修彬, 等. Multi-Latex多聚粒技术联合检测尿微量蛋白在肾移植术后无创诊断中的应用价值[J]. 器官移植, 2020, 11(4): 443-448. doi: 10.3969/j.issn.1674-7445.2020.04.003
Li Shengbing, Chen Wen, Li Xiubin, et al. The application value of Multi-Latex polygranular technique joint detection of urinary microproteins in noninvasive diagnosis after renal transplantation[J]. ORGAN TRANSPLANTATION, 2020, 11(4): 443-448. doi: 10.3969/j.issn.1674-7445.2020.04.003
Citation: Li Shengbing, Chen Wen, Li Xiubin, et al. The application value of Multi-Latex polygranular technique joint detection of urinary microproteins in noninvasive diagnosis after renal transplantation[J]. ORGAN TRANSPLANTATION, 2020, 11(4): 443-448. doi: 10.3969/j.issn.1674-7445.2020.04.003

Multi-Latex多聚粒技术联合检测尿微量蛋白在肾移植术后无创诊断中的应用价值

doi: 10.3969/j.issn.1674-7445.2020.04.003
基金项目: 

国家自然科学基金面上项目 81571555

军队医学科技青年培育计划 18QNP035

详细信息
    作者简介:

    李圣冰,女,1996年生,硕士研究生,Email:15230006363@163.com

    通讯作者:

    肖漓,女,1972年生,博士,主任技师,硕士研究生导师,Email:xiaolilab309@163.com

  • 中图分类号: R617, R692.5

The application value of Multi-Latex polygranular technique joint detection of urinary microproteins in noninvasive diagnosis after renal transplantation

More Information
  • 摘要:   目的  探讨Multi-Latex多聚粒技术联合检测肾损伤相关尿微量蛋白在肾移植术后无创诊断中的应用价值。  方法  回顾性分析72例肾移植受者的临床资料,根据血清肌酐(Scr)水平,分为肾功能正常组(A组,14例);肾功能轻度受损组(B组,37例);肾功能重度受损组(C组,21例);选取20例健康志愿者作为健康对照组(HC组)。采用Multi-Latex多聚粒技术检测上述各组研究对象的尿视黄醇结合蛋白(RBP)、微量白蛋白(mAlb)、IgG、转铁蛋白(TRF)、α1-微球蛋白(MG)和β2-MG含量。分析尿微量蛋白与Scr、血尿素氮(BUN)的相关性,比较各组尿微量蛋白差异,评价单一及联合检测尿微量蛋白的诊断价值。  结果  HC组和A组的6种尿微量蛋白均显著低于B组和C组,B组的6种尿微量蛋白均显著低于C组(均为P < 0.01)。肾移植受者的6种尿微量蛋白均与BUN呈正相关,RBP、mAlb、α1-MG和β2-MG均与Scr呈正相关,相关性均有统计学意义(P < 0.001~0.05)。尿微量蛋白联合检测的诊断价值优于单一指标的检测,其中TRF+mAlb+RBP+α1-MG四联检测诊断价值最高。  结论  6种尿微量蛋白可作为反映移植肾功能的特异性指标,多聚粒技术可同时检测其含量,实现无创诊断。基于TRF+mAlb+RBP+α1-MG四联检测的诊断有望进一步完善肾移植术后的无创诊断体系。

     

  • 图  1  4组研究对象尿微量蛋白含量的比较

    注:与HC组比较,aP < 0.01;与A组比较,bP < 0.01;与B组比较,cP < 0.01。

    Figure  1.  Comparison of the contents of urine microproteins of participants among 4 groups

    表  1  各项指标对移植肾功能状态的诊断价值比较

    Table  1.   Comparison of the diagnostic value of various indicators on the functional status of transplant kidney

    参数 AUC 95%可信区间 最佳诊断值(mg/L) 灵敏度 特异度
    单一检测
       TRF 0.932 0.875~0.989 0.795 0.828 1.000
      mAlb 0.913 0.846~0.981 8.850 0.879 0.857
      IgG 0.890 0.817~0.964 5.290 0.810 1.000
      RBP 0.903 0.834~0.973 0.233 0.776 1.000
      α1-MG 0.985 0.961~1.000 11.370 1.000 0.862
      β2-MG 0.862 0.777~0.946 0.145 0.845 0.929
    联合检测
      TRF+mAlb 0.932 0.875~0.990 1.000 0.929
      RBP+α1-MG 0.985 0.961~1.000 1.000 0.929
      RBP+α1-MG+β2-MG 0.984 0.959~1.000 1.000 0.857
      TRF+mAlb+IgG 0.935 0.879~0.990 1.000 0.929
      TRF+mAlb+RBP+α1-MG 0.989 0.970~1.000 1.000 0.929
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出版历程
  • 收稿日期:  2020-04-18
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2020-07-15

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